Unique and Cross-Reactive T Cell Epitope Peptides of the Major Bahia Grass Pollen Allergen, Pas n 1
Unique and Cross-Reactive T Cell Epitope Peptides of the Major Bahia Grass Pollen Allergen, Pas n 1
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DOI：
10.1159/000338290
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发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Rolland, Jennifer M.
中科院分区：
文献类型：
--
作者：
Etto, Tamara;de Boer, Carmela;Rolland, Jennifer M.
背景：巴伊亚草花粉（BaGP）是引起变应性鼻炎的主要原因。皮下变应原特异性免疫治疗对草花粉过敏有效，但由于过敏反应的风险，不适合中度至重度哮喘患者。T细胞反应性但IgE非反应性肽提供了更安全的治疗选择。本研究旨在鉴定和表征主要BaGP过敏原Pas n 1的显性CD4（+）T细胞表位肽。研究方法：Pas n 1特异性T细胞系产生的外周血的BaGP过敏的受试者进行了测试的增殖和细胞因子的反应重叠的20聚体Pas n 1肽。交叉反应同源肽从Lol p 1和Cyn d 1的黑麦草和百慕大草花粉，分别进行了评估，使用Pas n 1肽特异性T细胞克隆。通过HLA阻断试验测定Pas n 1肽T细胞识别的MHC II类限制，并通过斑点印迹法测定肽IgE反应性。结果：三个Pas n 1肽显示占主导地位的T细胞反应性，15 18例（83%）的患者响应一个或多个这些肽。特异性占主导地位的Pas n 1肽的T细胞克隆显示出物种特异性T细胞反应性以及与其他组1草花粉过敏原的交叉反应性的证据。占优势的Pas n 1 T细胞表位肽显示HLA结合多样性，并且不与IgE反应。结论：免疫显性T细胞反应性Pas n 1肽是个体安全免疫治疗的候选者，包括对巴伊亚和可能的其他草花粉过敏的哮喘患者。版权所有（C）2012 S. Karger AG，巴塞尔
Background: Bahia grass pollen (BaGP) is a major cause of allergic rhinitis. Subcutaneous allergen-specific immunotherapy is effective for grass pollen allergy, but is unsuitable for patients with moderate to severe asthma due to the risk of anaphylaxis. T cell-reactive but IgE nonreactive peptides provide a safer treatment option. This study aimed to identify and characterize dominant CD4(+) T cell epitope peptides of the major BaGP allergen, Pas n 1. Methods: Pas n 1-specific T cell lines generated from the peripheral blood of BaGP-allergic subjects were tested for proliferative and cytokine response to overlapping 20-mer Pas n 1 peptides. Cross-reactivity to homologous peptides from Lol p 1 and Cyn d 1 of Ryegrass and Bermuda grass pollen, respectively, was assessed using Pas n 1 peptide-specific T cell clones. MHC class II restriction of Pas n 1 peptide T cell recognition was determined by HLA blocking assays and peptide IgE reactivity tested by dot blotting. Results: Three Pas n 1 peptides showed dominant T cell reactivity; 15 of 18 (83%) patients responded to one or more of these peptides. T cell clones specific for dominant Pas n 1 peptides showed evidence of species-specific T cell reactivity as well as cross-reactivity with other group 1 grass pollen allergens. The dominant Pas n 1 T cell epitope peptides showed HLA binding diversity and were non-IgE reactive. Conclusions: The immunodominant T cell-reactive Pas n 1 peptides are candidates for safe immunotherapy for individuals, including those with asthma, who are allergic to Bahia and possibly other grass pollens. Copyright (C) 2012 S. Karger AG, Basel