Spatio-temporal expression of PsAPY1 during germination, defferentiation, and organogenesis in pea (Pisum sativum L. Var. Alaska)
Spatio-temporal expression of PsAPY1 during germination, defferentiation, and organogenesis in pea (Pisum sativum L. Var. Alaska)
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豌豆 (Pisum sativum L. Var. Alaska) 发芽、分化和器官发生过程中 PsAPY1 的时空表达
DOI：
10.1007/s11738-012-1166-9
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Abe S.
中科院分区：
文献类型：
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作者：
Trivima T. Sahi V.P.;Morita E.H.;Abe S.
Apyrase （atp -二磷酸水解酶，EC 3.6.1.5）催化核苷三磷酸和二磷酸水解为核苷单磷酸和正磷酸。本研究对豌豆（PisumsativumL）中apy酶基因（PsAPY1）的时空表达进行了研究。在萌发早期[吸胀后10 h和播种后16、35、62和84 h]，利用非放射性mRNA原位杂交技术对阿拉斯加种的种子进行了研究。在吸胀后10 h， psapy1转录本在胚的茎和根组织中可见。16个HAS在茎尖分生组织、表皮、维管束和根尖的被膜层中转录本积累量增加。35 HAS时，表达信号在生长期茎尖区和根的体组织中分布更广、更强；在表皮和分化维管束中表达量最高，在皮层组织中表达量略低。到62个HAS时，psapy1的表达最强，且广泛分布在上述区域。在上胚轴向胚珠和钩分化的区域也有较高的表达。然而，在84 HAS时，psapy1在茎和根中的表达下降，但在生长侧根原基中表达高。幼苗的萌发和生长是植物生命周期中最重要的生理阶段。psapy1在萌发、分化和器官发生等重要阶段的存在表明其在植物生长发育中的重要作用。
Apyrase (ATP-diphosphohydrolase, EC 3.6.1.5) catalyses the hydrolysis of nucleoside tri- and di-phosphates to nucleoside monophosphates and orthophosphates. In the present study, the spatio-temporal expression of an apyrase gene (PsAPY1)from pea (PisumsativumL. Var. Alaska) was investigated during the early stages of germination [10 h after imbibition and 16, 35, 62, and 84 h after sowing (HAS)], using non-radioactive mRNA in situ hybridization. At 10 h after imbibition, thePsAPY1transcript was visible in traces throughout the shoot and root tissues of the embryo. The transcript accumulation became higher by 16 HAS in the tunica layer of the shoot apical meristem, epidermis, vascular bundle, and root tip. At 35 HAS, expression signals became stronger and more widely distributed in the corpus tissues of the growing shoot apical region and in the root; the highest expression was detected in the epidermis, differentiating vascular bundle, and somewhat less in cortex tissues. By 62 HAS, the expression ofPsAPY1was strongest and extensively distributed in the above mentioned regions. At this time, higher expression was also detected in the zones of epicotyl differentiating into plumule and hook. However, at 84 HAS, the expression ofPsAPY1declined in the shoot and root, but was highly expressed in growing lateral root primordia. Germination and growth of seedlings are the physiologically most important stages in the life cycle of plants. The presence ofPsAPY1during these important stages i.e., germination, differentiation, and organogenesis indicates its essential role in growth and development of plants.