A Planarian Model System to Study Host-Pathogen Interactions.
A Planarian Model System to Study Host-Pathogen Interactions.
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DOI：
10.1007/978-1-0716-3275-8_14
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发表时间：
2023
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本研究利用新近建立的真涡虫感染模型系统研究真菌感染过程中宿主-病原体的相互作用。在这里，我们详细描述了感染的涡虫Schmidtea mediorea与人类真菌病原体白色念珠菌。这种简单且可重复的模型系统允许在不同感染时间点快速可视化组织损伤。我们注意到，这个模型系统已经针对与C一起使用进行了优化。白色念珠菌，但也应该适用于与其他感兴趣的病原体一起使用。
This protocol is focused on using the recently established planarian infection model system to study host-pathogen interactions during fungal infection. Here, we describe in detail the infection of the planarian Schmidtea mediterranea with the human fungal pathogen Candida albicans. This simple and reproducible model system allows for rapid visualization of tissue damage throughout different infection timepoints. We note that this model system has been optimized for use with C. albicans, but should also be applicable for use with other pathogens of interest.