Alterations in metal toxicity and metal-induced metallothionein gene expression elicited by growth medium calcium concentration.
Alterations in metal toxicity and metal-induced metallothionein gene expression elicited by growth medium calcium concentration.
复制标题
生长培养基钙浓度引起金属毒性和金属诱导的金属硫蛋白基因表达的改变。
DOI：
10.1007/s10565-007-9036-8
复制
发表时间：
2008
影响因子：
6.1
通讯作者：
Garrett,ScottH
中科院分区：
文献类型：
--
作者：
Singh,RajendraK;Albrecht,AmyL;Somji,Seema;Sens,MaryAnn;Sens,DonaldA;Garrett,ScottH
生长培养基的钙含量已显示影响培养物中原代上皮细胞的生长和分化。本研究的目的是确定生长培养基中的钙浓度是否会影响永生化的人前列腺源性上皮细胞系（RWPE-1）对金属毒性和金属硫蛋白基因表达的易感性。RWPE-1细胞系在含有0.1或1.4 mM钙的培养基中生长。将融合细胞暴露于Zn+2（50、100或150 μM）或Cd+2（3、6或12 μM）13天，并测定沿着暴露时间的细胞毒性和MT基因表达。结果表明，培养基中钙含量对Zn+2的毒性有显著影响，对Cd+2的毒性影响较小，但有显著性差异。生长培养基的钙浓度也显示出改变MT-1/2蛋白和MT-1 E、MT-1X和MT-2A mRNA的积累。结果表明，MT-1/2蛋白在含0.1mM钙的培养基中生长的金属暴露细胞中显著增加，然而，表达的增加并不引起细胞对Zn+2或Cd+2暴露的抗性增加。这些观察结果表明，生长培养基钙浓度可影响金属毒性以及RWPE-1细胞的MT mRNA和蛋白质的表达模式。结果表明，当比较可能在钙浓度不同的生长制剂中生长的细胞系之间的毒理学反应时，应谨慎行事。
The calcium content of the growth medium has been shown to influence the growth and differentiation of primary epithelial cells in culture. The goal of the present study was to determine if growth medium calcium concentration could influence the susceptibility to metal toxicity and metallothionein gene expression of an immortalized human prostate-derived epithelial cell line (RWPE-1). The RWPE-1 cell line was grown in medium containing either 0.1 or 1.4 mM calcium. Confluent cells were exposed to either Zn+2(50, 100, or 150 μM) or Cd+2(3, 6, or 12 μM) for 13 days, and cell toxicity and MT gene expression were determined along the time course of exposure. It was demonstrated that the calcium content of the growth medium had a marked influence on Zn+2toxicity and a lesser but significant effect on Cd+2toxicity to the RWPE-1 cells. Calcium concentration of the growth medium was also shown to alter the accumulation of MT-1/2 protein and MT-1E, MT-1X, and MT-2A mRNAs. It was shown that MT-1/2 protein was markedly increased for metal-exposed cells grown in medium containing 0.1 mM calcium; however, the increased expression did not cause an increase in the resistance of the cells to Zn+2or Cd+2exposure. These observations show that growth medium calcium concentration can influence metal toxicity and the pattern of expression of the MT mRNAs and protein for RWPE-1 cells. The results suggest that caution should be exercised when comparing toxicological responses between cell lines that may be grown in growth formulations differing in calcium concentration.