Increased expression of dipeptidyl peptidase IV in human mesothelial cells by malignant ascites from ovarian carcinoma patients
Increased expression of dipeptidyl peptidase IV in human mesothelial cells by malignant ascites from ovarian carcinoma patients
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DOI：
10.1159/000063801
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发表时间：
2002-01-01
期刊：
影响因子：
3.5
通讯作者：
Mizutani, S
中科院分区：
文献类型：
--
作者：
Kajiyama, H;Kikkawa, F;Mizutani, S
细胞表面氨肽酶通过降解小分子肽在生物过程中发挥重要作用。腹水中含有多种活性肽，这些活性肽参与了癌细胞的播散和浸润。在人间皮细胞中，二肽基肽酶IV（DPPIV）主要在四种细胞表面氨基肽酶中显示最高表达：氨基肽酶A、中性内肽酶24-11、氨基肽酶N和DPPIV。由于间皮细胞总是与腹水接触，我们研究了恶性腹水对DPPIV的影响。DPPIV酶活性在mesothelium细胞中的增强，通过添加从卵巢癌患者中获得的腹水中的时间和浓度依赖性的方式，流式细胞术和免疫细胞化学也揭示了DPPIV在mesothelium细胞的细胞表面上的表达增加。的
Cell surface aminopeptidases play an important role in biological processes through degradation of small peptides. There are many bioactive peptides in ascites and these peptides are involved in carcinoma cell dissemination and infiltration. In human mesothelial cells dipeptidyl peptidase IV (DPPIV) shows the highest expression mostly in four cell surface aminopeptidases: aminopeptidase A, neutral endopeptidase 24-11, aminopeptidase N and DPPIV. Since mesothelial cells are always in contact with ascites, we examined the influence of malignant ascites on DPPIV. DPPIV enzyme activity in mesothelial cells was enhanced by the addition of ascites obtained from ovarian carcinoma patients in a time- and concentration-dependent manner, and flow cytometry and immunocytochemistry also revealed an increased expression of DPPIV on the cell surface of mesothelial cells. The