Cognitive frailty predicting death and disability in Chinese elderly
Cognitive frailty predicting death and disability in Chinese elderly
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DOI：
10.1080/01616412.2021.1939235
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发表时间：
2021-07-25
影响因子：
1.9
通讯作者：
Li, Shujuan
中科院分区：
文献类型：
--
作者：
Ma, Yajun;Li, Xiaodong;Li, Shujuan
目的：虽然多发性硬化症（MS）是一种已知的免疫介导的疾病，但对其发病机制知之甚少。MicroRNAs （miRNAs）是参与基因表达调控的小非编码蛋白。神经退行性疾病中的t细胞活化电位是近年来关注的研究课题，细胞因子在MS的病程和发病机制中发挥着重要作用，本研究旨在分析靶向细胞因子IL-17和IL-23的miR-20、miR-21、miR-26、miR-155和Let-7的表达水平，以评估MS与调节自身免疫通路中细胞因子表达的mirna之间的关系。方法：该研究包括20例复发缓解型多发性硬化症（MS）患者，年龄至少18岁，正在接受门诊免疫调节治疗，以及20例健康，无全身性疾病且未服用任何药物的无关个体作为对照组。将所有参与者的外周血样本收集到含有edta的试管中，分离血浆用于cDNA合成。从这些cDNA样本中，使用实时PCR装置中的miScript SYBR Green试剂盒，通过熔化曲线分析定量分析miRNA表达水平。结果：将MS患者和健康人外周血样本中的miRNA表达水平进行比较发现，MS患者miR-20表达水平明显上调，miR-26和miR-155表达水平明显下调(p
Objective: Although multiple sclerosis (MS) is known to be an immune-mediated disease, very little is known about its etiopathogenesis. MicroRNAs (miRNAs) are small non-coding proteins involved in the regulation of gene expression. T-cell activation potential in neurodegenerative diseases has been a research topic of interest in recent years Cytokines play an important role in the course and pathogenesis of MS, The aim of the present study was to analyze expression levels of miR-20, miR-21, miR-26, miR-155, and Let-7, which target the cytokines interleukin IL-17 and IL-23, in order to evaluate the relationship between MS and miRNAs that modulate the expression of cytokines involved in the autoimmune pathway. Methods:The study included 20 relapsing-remitting multiple sclerosis (MS) patients who were at least 18 years of age and were undergoing outpatient immunomodulatory therapy and 20 healthy, unrelated individuals who had no systemic disease and were not taking any medication as a control group. Peripheral blood samples were collected from all participants into EDTA-containing tubes and plasma was isolated for cDNA synthesis . From these cDNA samples, miRNA expression levels were quantitatively analyzed via melting curve analysis using the miScript SYBR Green kit in real-time PCR device. Results: Comparison of miRNA expression levels in the peripheral blood samples and MS patients and healthy subjects revealed that the MS patients had significant upregulation of miR-20 and downregulation of miR-26 and miR-155 compared to the control group (p