Neural induction by the node and placode induction by head mesoderm share an initial state resembling neural plate border and ES cells.
Neural induction by the node and placode induction by head mesoderm share an initial state resembling neural plate border and ES cells.
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节点的神经诱导和头部中胚层的基板诱导共享类似于神经板边界和ES细胞的初始状态。
DOI：
10.1073/pnas.1719674115
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发表时间：
2018
影响因子：
11.1
通讯作者：
Trevers KE
中科院分区：
文献类型：
--
作者：
Trevers KE
在高等脊椎动物胚胎原肠胚形成前后，诱导相互作用引导细胞形成中枢神经系统（神经板）或感觉基板。将不同的组织移植到鸡胚胎的外周会引起不同的反应：亨森结诱导神经板，而头部中胚层诱导基板。这些过程有何不同？时间进程中的转录组分析显示，这两个过程都是从诱导一组共同的基因开始的，这些基因后来出现分歧。这些基因与胚外组织诱导的基因非常相似，通常在原肠胚形成前的上胚层中表达。在没有进一步信号的情况下生长的外胚层的外植体发育为神经板边缘衍生物，并最终表达透镜标记。我们将这种状态称为“前边界”;它的转录组类似于胚胎干细胞。最后，使用顺序移植实验，我们表明，节点，头部中胚层和下胚层是可互换的开始任何这些诱导，而最终的结果取决于组织发射后来的信号。
Around the time of gastrulation in higher vertebrate embryos, inductive interactions direct cells to form central nervous system (neural plate) or sensory placodes. Grafts of different tissues into the periphery of a chicken embryo elicit different responses: Hensen’s node induces a neural plate whereas the head mesoderm induces placodes. How different are these processes? Transcriptome analysis in time course reveals that both processes start by induction of a common set of genes, which later diverge. These genes are remarkably similar to those induced by an extraembryonic tissue, the hypoblast, and are normally expressed in the pregastrulation stage epiblast. Explants of this epiblast grown in the absence of further signals develop as neural plate border derivatives and eventually express lens markers. We designate this state as “preborder”; its transcriptome resembles embryonic stem cells. Finally, using sequential transplantation experiments, we show that the node, head mesoderm, and hypoblast are interchangeable to begin any of these inductions while the final outcome depends on the tissue emitting the later signals.