Behavioral responses to anxiogenic tasks in young adult rats with neonatal dopamine depletion
Behavioral responses to anxiogenic tasks in young adult rats with neonatal dopamine depletion
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新生多巴胺耗竭的年轻成年大鼠对致焦虑任务的行为反应
DOI：
10.1016/j.physbeh.2019.02.006
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发表时间：
2019
影响因子：
2.9
通讯作者：
Ishibashi H
中科院分区：
文献类型：
--
作者：
Ogata M;Akita H;Ishibashi H
多巴胺能神经系统在运动调节和焦虑相关行为的调节中起着至关重要的作用。虽然新生儿期多巴胺缺乏的大鼠表现出运动多动，并已被用作注意缺陷多动障碍的动物模型，但缺乏对它们在引发焦虑的条件下的行为特征的描述。在本研究中，我们采用旷场、高架迷宫和明暗(L/B)盒子测试，研究了新生大鼠多巴胺缺乏时对焦虑性刺激的行为反应。OF和EPM测试在弱光和强光条件下进行。同时观察甲基苯丙胺(MAP)或托莫西汀(ATX)对新生儿多巴胺缺乏所致行为异常的改善作用。出生后第4天接受6-羟基多巴胺治疗的大鼠，在两种条件下的测试和在强光条件下的 测试中，运动活动显著增加，焦虑相关行为减少。此外，新生儿期多巴胺缺乏的大鼠对焦虑刺激强度的变化没有表现出正常的行为反应。MAP(4 mg/kg)和ATX(1.2 mg/kg/d)可改善运动多动，但不能改善焦虑相关的异常行为。这些结果表明，多巴胺能系统在涉及运动和焦虑相关行为的神经网络的发展中起着至关重要的作用。结果表明，焦虑反应异常的机制与运动多动的机制部分不同。
The dopaminergic neural system plays a crucial role in motor regulation as well as regulation of anxiety-related behaviors. Although rats with neonatal dopamine depletion exhibit motor hyperactivity and have been utilized as animal models of attention deficit hyperactivity disorder, characterization of their behavior under anxiogenic conditions is lacking. In the present study, we investigated behavioral responses to anxiogenic stimuli in young adult rats with neonatal dopamine depletion using the open field (OF), elevated plus maze (EPM), and light/dark (L/B) box tests. The OF and EPM tests were performed under low-light and bright-light conditions. The ameliorative effects of pretreatment with methamphetamine (MAP) or atomoxetine (ATX) on abnormal behaviors induced by neonatal dopamine depletion were also assessed. Rats that underwent 6-hydroxydopamine treatment 4 day after birth showed significant increases in motor activity and decreases in anxiety-related behaviors in OF tests under both conditions and in EPM tests under bright-light conditions. Furthermore, rats with neonatal dopamine depletion did not show normal behavioral responsiveness to changes in the intensity of anxiogenic stimuli. Pretreatment with MAP (4 mg/kg) and ATX (1.2 mg/kg/day) ameliorated motor hyperactivity but not abnormal anxiety-related behaviors. These results suggest that the dopaminergic system plays a crucial role in the development of neural networks involved in locomotion as well as in those involved in anxiety-related behavior. The results indicate that the mechanisms underlying the abnormal anxiolytic responses partially differ from those underlying motor hyperactivity.