Interaction of human gallbladder mucin with calcium hydroxyapatite: Binding studies and the effect on hydroxyapatite formation
Interaction of human gallbladder mucin with calcium hydroxyapatite: Binding studies and the effect on hydroxyapatite formation
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DOI：
10.1002/hep.1840210621
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发表时间：
1995-06
期刊：
影响因子：
13.5
通讯作者：
S. Qiu;G. Wen;J. Wen;R. Soloway;R. Crowther
中科院分区：
文献类型：
--
作者：
S. Qiu;G. Wen;J. Wen;R. Soloway;R. Crowther
在聚合的人胆囊粘蛋白（1.0 mg/mL）存在下，体外形成的羟基磷灰石（HAP）晶体（0.75 ± 0.39 μm）比对照晶体（7.86 ± 2.76 μm）小，但粘蛋白不影响晶体形成的动力学或改变平衡时存在的矿物相的量。与此相反，天然粘蛋白的蛋白水解产生的糖肽亚基对HAP晶体大小没有影响。天然粘蛋白和糖肽都与成熟的HAP晶体结合，但糖肽更容易被磷酸根离子取代。因此，在HAP形成的实验中，磷酸根离子抑制了糖肽与新生HAP的相互作用。这些结果表明，胆囊粘蛋白可以调节HAP的形成在体内，这种能力可能会改变在病理状态，如中性粒细胞浸润或细菌定植，这可能会导致能够消化粘蛋白的蛋白酶的释放。
Calcium hydroxyapatite (HAP) crystals formed in vitro in the presence of polymeric human gallbladder mucin (1.0 mg/mL) were smaller (0.75 ± 0.39 μm) than control crystals (7.86 ± 2.76 μm), but the mucin did not affect the kinetics of crystal formation or alter the amount of mineral phase present at equilibrium. In contrast, glycopeptide subunits produced by proteolysis of the native mucin had no effect on HAP crystal size. Both native mucin and glycopeptides bound to mature HAP crystals, but the glycopeptides were much more readily displaced by phosphate ions. Therefore, in experiments where HAP was being formed, the phosphate ions inhibited the interaction of glycopeptides with the nascent HAP. These results indicate that gallbladder mucin may modulate HAP formation in vivo, and that this ability may be altered during pathological states, such as neutrophil infiltration or bacterial colonization, that may cause the release of proteinases capable of digesting mucin.