Induction of nitrate reductase and membrane cytochromes in wild type and chlorate-resistant Paracoccus denitrificans.
Induction of nitrate reductase and membrane cytochromes in wild type and chlorate-resistant Paracoccus denitrificans.
复制标题
野生型和耐氯酸盐脱氮副球菌中硝酸还原酶和膜细胞色素的诱导。
DOI：
10.1007/bf00511220
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发表时间：
1980
影响因子：
2.8
通讯作者：
Lascelles,J
中科院分区：
文献类型：
--
作者：
Calder,K;Burke,KA;Lascelles,J
本文设计了一个实验系统，在叠氮化物、硝酸盐或亚硝酸盐存在下，在有限通气条件下诱导野生型嗜热副球菌悬浮液中硝酸还原酶。叠氮促进酶的最大合成，伴随着过量的b型细胞色素的形成;硝酸盐获得的酶的水平较少，c型细胞色素占主导地位的膜。硝酸还原酶溶解与脱氧胆酸盐从叠氮化物诱导的细胞膜，并确定为一个主要的polypeptideMr= 150，000十二烷基硫酸钠-聚丙烯酰胺凝胶电泳。根据对氯酸盐的抗性，分离出缺乏硝酸还原酶活性的突变株，并详细检查了突变株M-1。当在细胞悬浮系统中孵育时，M-1形成膜蛋白Mr = 150，000，与野生型中归因于硝酸还原酶的膜蛋白相似。在没有诱导剂的情况下，M-1的蛋白质形成量最大，并伴随着过量b型细胞色素的合成。对野生型和M-1的观察表明，硝酸还原酶蛋白和b型细胞色素是共调节的，活性酶在调节其自身的合成中起作用。
An experimental system has been devised for induction of nitrate reductase in suspensions of wild typeParacoccus denitrificansincubated with limited aeration in the presence of azide, nitrate or nitrite. Azide promoted maximum synthesis of enzyme, accompanied by formation of excessb-type cytochrome; the level of enzyme attained with nitrate was less andc-type cytochrome predominated in the membrane. The nitrate reductase was solubilized with deoxycholate from membranes of azide-induced cells and was identified as a major polypeptideMr=150,000 by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Mutants strains lacking nitrate reductase activity were isolated on the basis of resistance to chlorate and mutant M-1 was examined in detail. When incubated in the cell suspension system M-1 formed a membrane proteinMr=150,000 similar to that attributed to nitrate reductase in the wild type. Maximum formation of the protein by M-1 occurred without inducer and it was accompanied by synthesis of excessb-type cytochrome. The observations with wild type and M-1 indicate that nitrate reductase protein andb-type cytochrome are coregulated and that the active enzyme has a role in regulating its own synthesis.