Antibodies to tissue transglutaminase C in type I diabetes
Antibodies to tissue transglutaminase C in type I diabetes
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DOI：
10.1007/s001250051291
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发表时间：
1999-10-01
期刊：
影响因子：
8.2
通讯作者：
Bonifacio, E
中科院分区：
文献类型：
--
作者：
Lampasona, V;Bonfanti, R;Bonifacio, E
目的/假设。无症状性乳糜泻是一种麸质驱动的自身免疫性疾病，在I型（胰岛素依赖型）糖尿病患者中相对常见。为了确定I型糖尿病中谷蛋白相关自身免疫的程度，检测了新发I型糖尿病患者的组织转氨酶C（乳糜泻中的主要自身抗原）自身抗体。我们使用人重组抗原和放射结合试验检测了287例新发I型糖尿病患者、119例II型糖尿病患者和213例对照组患者的IgG和伊加组织转氨酶C自身抗体。我们发现伊加和IgG组织转氨酶C抗体在24例（8%）I型糖尿病患者，97例（33%）患者有IgG抗体和1伊加抗体。抗体浓度最高的伊加和IgG抗体。仅2例（2%）II型糖尿病患者和2例（1%）对照受试者有IgG或伊加组织转氨酶C抗体。HLA-DRB 1 *04等位基因的患者IgG组织转氨酶C抗体的患病率最高。这些数据表明，几乎10%的患者具有腹腔疾病的典型自身免疫性，另外30%具有低水平的组织转氨酶C抗体结合。这种高患病率表明肠道参与I型糖尿病的发病机制，或者转氨酶是β细胞破坏引起的继发性自身抗原。
Aims/hypothesis. Silent coeliac disease is a gluten driven autoimmune disease which is relatively frequent in patients with Type I (insulin-dependent) diabetes mellitus. To determine the extent of gluten associated autoimmunity in Type I diabetes, autoantibodies to tissue transglutaminase C, a major autoantigen in coeliac disease, were measured in patients with new-onset Type I diabetes.Methods. We measured IgG and IgA tissue transglutaminase C autoantibodies using human recombinant antigen and radio-binding assays in a cohort of 287 patients with new-onset Type I diabetes, 119 with Type II (non-insulin-dependent) diabetes mellitus and in 213 control subjects.Results. We found IgA and IgG tissue transglutaminase C antibodies in 24 (8%) patients with Type I diabetes; 97 (33%) patients had IgG antibodies only and 1 IgA antibodies only. Antibody concentrations were highest in those with both IgA and IgG antibodies. Only 2 (2%) patients with Type II diabetes and 2 (1%) control subjects had either IgG or IgA tissue transglutaminase C antibodies. Patients with HLA DRB1*04 alleles had the highest prevalence of IgG tissue transglutaminase C antibodies.Conclusion/Interpretation. These data show that almost 10% of patients have autoimmunity typical of coeliac disease and that another 30% have low level tissue transglutaminase C antibody binding. This high prevalence suggests either involvement of the gut in the pathogenesis of Type I diabetes or that transglutaminase is a secondary autoantigen resulting from beta-cell destruction.