Generation of an Avian-Mammalian Rotavirus Reassortant by Using a Helper Virus-Dependent Reverse Genetics System
Generation of an Avian-Mammalian Rotavirus Reassortant by Using a Helper Virus-Dependent Reverse Genetics System
复制标题
DOI：
10.1128/jvi.02730-15
复制
发表时间：
2016-02-01
影响因子：
5.4
通讯作者：
Trojnar, Eva
中科院分区：
文献类型：
--
作者：
Johne, Reimar;Reetz, Jochen;Trojnar, Eva
轮状病毒 A (RVA) 株的遗传多样性部分是通过遗传重配而促进的。尽管这种基因组片段交换过程在哺乳动物 RVA 中经常被报道，但哺乳动物 RVA 是否也可以包装禽类 RVA 品系的基因组片段仍然未知。我们生成了含有鸡 RVA 品系 02V0002G3 的 VP4 基因的猿 RVA 品系 SA11 重配体。为了实现这一目标，我们用T7聚合酶驱动的VP4编码质粒转染BSR5/T7细胞，用温度敏感的SA11 VP4突变体感染细胞，并通过升高温度来选择重组病毒。重配病毒可以在MA-104细胞中稳定传代并表现出细胞病变效应，但其复制效率低于亲本病毒。我们的结果表明，鸟类和哺乳动物轮状病毒可以交换基因组片段，产生具有新基因组和抗原特征的复制能力重配体。
The genetic diversity of rotavirus A (RVA) strains is facilitated in part by genetic reassortment. Although this process of genome segment exchange has been reported frequently among mammalian RVAs, it remained unknown if mammalian RVAs also could package genome segments from avian RVA strains. We generated a simian RVA strain SA11 reassortant containing the VP4 gene of chicken RVA strain 02V0002G3. To achieve this, we transfected BSR5/T7 cells with a T7 polymerase-driven VP4-encoding plasmid, infected the cells with a temperature-sensitive SA11 VP4 mutant, and selected the recombinant virus by increasing the temperature. The reassortant virus could be stably passaged and exhibited cytopathic effects in MA-104 cells, but it replicated less efficiently than both parental viruses. Our results show that avian and mammalian rotaviruses can exchange genome segments, resulting in replication-competent reassortants with new genomic and antigenic features.