NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction.
NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction.
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DOI：
10.1093/jxb/erx047
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发表时间：
2017-03-01
影响因子：
6.9
通讯作者：
Hussey PJ
中科院分区：
文献类型：
--
作者：
Wang P;Hussey PJ
在植物细胞中表达高水平的GFP标记的NETWORKED 3B（NET 3B）增强了内质网和肌动蛋白细胞骨架之间的关联。植物内质网的运动依赖于肌动蛋白细胞骨架。然而，对连接内质网膜和肌动蛋白细胞骨架的蛋白质知之甚少。在这里，我们确定了一种新的蛋白质，NETWORKED 3B（NET 3B），这是与ER和肌动蛋白细胞骨架在体内。NET 3B属于植物特异性肌动蛋白结合蛋白的超家族，NETWORKED家族。NET 3B通过N-末端NET肌动蛋白结合（NAB）结构域与体内肌动蛋白细胞骨架相关联，该结构域在NET家族的其他成员中已得到充分表征。NET 3B的NAB结构域中的三个氨基酸插入Val-Glu-Asp似乎降低了其定位于肌动蛋白细胞骨架的能力，与NET家族的创始成员NET 1A相比。NET 3B的C-末端结构域将蛋白质连接到ER。NET 3B的过表达增强了ER和肌动蛋白细胞骨架之间的关联，这种关联的程度取决于可用的NET 3B的量。NET 3B过表达的另一个影响是ER膜扩散的减少。总之，我们的研究结果表明，NET 3B调节ER和肌动蛋白细胞骨架在高等植物中的相互作用。
Expressing high levels of GFP-tagged NETWORKED 3B (NET 3B) in plant cells enhances the association between the endoplasmic reticulum and actin cytoskeleton. In plants movement of the endoplasmic reticulum (ER) is dependent on the actin cytoskeleton. However little is known about proteins that link the ER membrane and the actin cytoskeleton. Here we identified a novel protein, NETWORKED 3B (NET3B), which is associated with the ER and actin cytoskeleton in vivo. NET3B belongs to a superfamily of plant specific actin binding proteins, the NETWORKED family. NET3B associates with the actin cytoskeleton in vivo through an N-terminal NET actin binding (NAB) domain, which has been well-characterized in other members of the NET family. A three amino acid insertion, Val-Glu-Asp, in the NAB domain of NET3B appears to lower its ability to localize to the actin cytoskeleton compared with NET1A, the founding member of the NET family. The C-terminal domain of NET3B links the protein to the ER. Overexpression of NET3B enhanced the association between the ER and the actin cytoskeleton, and the extent of this association was dependent on the amount of NET3B available. Another effect of NET3B overexpression was a reduction in ER membrane diffusion. In conclusion, our results revealed that NET3B modulates ER and actin cytoskeleton interactions in higher plants.