PENTAGASTRIN AND GASTRIC-MUCOSAL BLOOD-FLOW
PENTAGASTRIN AND GASTRIC-MUCOSAL BLOOD-FLOW
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DOI：
10.1152/ajpgi.1986.250.5.g575
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发表时间：
1986-05-01
影响因子：
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通讯作者：
BERGLINDH, T
中科院分区：
文献类型：
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作者：
HOLMRUTILI, L;BERGLINDH, T
应用活体显微镜观察了五肽胃泌素对大鼠胃粘膜微循环的影响。对浅表粘膜血管进行视频记录，用于红细胞速度（VRBC）和血管直径的离线分析，由此计算血流量（≥ QRBC）。从单个微血管流量数据计算静息粘膜血流量，并且血管分布为40 ml. min-1. 100 g-1。以20 μ g cntdot的剂量静脉输注五肽胃泌素。kg-1. h-1导致次最大的酸分泌（约0.0001）。60%）和QRBC显著增加47 ± 0.6%。百分之十四当以96 μ g·cm·dot的剂量给予时，kg-1. h-1静脉注射，导致最大的酸分泌和Orhdot.QRBC增加36 ± 0.5%。百分之十四在另一系列的实验中，在输注五肽胃泌素（20 μ g·cm·d）期间的QRBC测量结果显示在输注后的10天内。kg-1. h ~（-1）iv），并与氨基比林（AP）清除率或激光多普勒血流仪（LDF）进行比较。由[14 C]AP清除率测定的胃粘膜血流量增加309 ± 0.5%。115%，而QRBC增加了34 ± 0.1%。百分之十一当用LDF测定时，血流量增加了41 . ±. 22%，该值类似于QRBC的增加（50 ± 0.2%）。19%）。因此，在五肽胃泌素输注期间，通过AP清除率估计的血流量增加百分比显著高于通过直接微血管测量或LDF观察到的百分比。
The effect of pentagastrin on mucosal microcirculation was studied in rats by use of intravital microscopy. The superficial mucosal vessels were videorecorded for off-line analysis of red cell velocities (VRBC) and vessel diameters, from which blood flow (.ovrhdot.QRBC) was calculated. Resting mucosal blood flow calculated from single microvascular flow data, and vessel distribution was 40 ml .cntdot. min-1 .cntdot. 100 g-1. Pentagastrin infused intravenously in a dose of 20 .mu.g .cntdot. kg-1 .cntdot. h-1 resulted in submaximal acid secretion (.apprx. 60%) and a significant increase in .ovrhdot.QRBC by 47 .+-. 14%. When given in a dose of 96 .mu.g .cntdot. kg-1 .cntdot. h-1 iv, it resulted in maximal acid secretion and an increase in .ovrhdot.QRBC by 36 .+-. 14%. In another series of experiments the results of .ovrhdot.QRBC measurements during infusion of pentagastrin (20 .mu.g .cntdot. kg-1 .cntdot. h-1 iv) were compared with those of aminopyrine (AP) clearance or laser-Doppler flowmetry (LDF) in the same animals. Gastric mucosal blood flow determined by [14C]AP clearance increased by 309 .+-. 115%, whereas .ovrhdot.QRBC increased by 34 .+-. 11%. When determined by LDF, blood flow increased by 41 .+-. 22%, a value similar to the increase in .ovrhdot.QRBC (50 .+-. 19%). Thus, the percent increase in blood flow during pentagastrin infusion estimated by AP clearance was considerably higher than that observed by either direct microvascular measurements or by LDF.