Gene expression patterns in renal cell carcinoma assessed by complementary DNA microarray
Gene expression patterns in renal cell carcinoma assessed by complementary DNA microarray
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DOI：
10.1016/s0002-9440(10)63887-4
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发表时间：
2003-03-01
影响因子：
6
通讯作者：
Brooks, JD
中科院分区：
文献类型：
--
作者：
Higgins, JPT;Shinghal, R;Brooks, JD
肾细胞癌包括几种具有不同临床行为的组织类型。准确的病理特征在这些肿瘤的临床治疗中是重要的。我们描述了41个肾脏肿瘤的基因表达谱，这些基因表达谱是通过DNA微阵列确定的，该微阵列包含22,648个独特的cDNA，代表17,083个不同的Unigene簇，包括7230个特征人类基因。不同肿瘤类型之间的基因表达模式的差异是显而易见的；肿瘤样本的等级聚类分析仅根据其基因表达模式来区分组织上不同的肿瘤类型。传统的透明细胞肾癌表现出高度独特的基因表达模式。乳头状癌形成了一个紧密聚集的组，来自远端肾单位和正常肾样本的肿瘤也是如此。令人惊讶的是，具有颗粒状细胞质的传统肾癌是异质性的，在基因表达模式上与任何具有透明细胞质的传统癌都不相似。基于基因表达模式的肾癌特征提供了对这些肿瘤的修订分类，并有可能提供重要的生物学和临床见解。
Renal cell carcinoma comprises several histological types with different clinical behavior. Accurate pathological characterization is important in the clinical management of these tumors. We describe gene expression profiles in 41 renal tumors determined by using DNA microarrays containing 22,648 unique cDNAs representing 17,083 different UniGene Clusters, including 7230 characterized human genes. Differences in the patterns of gene expression among the different tumor types were readily apparent; hierarchical cluster analysis of the tumor samples segregated histologically distinct tumor types solely based on their gene expression patterns. Conventional renal cell carcinomas with clear cells showed a highly distinctive pattern of gene expression. Papillary carcinomas formed a tightly clustered group, as did tumors arising from the distal nephron and the normal kidney samples. Surprisingly, conventional renal cell carcinomas with granular cytoplasm were heterogeneous, and did not resemble any of the conventional carcinomas with clear cytoplasm in their pattern of gene expression. Characterization of renal cell carcinomas based on gene expression patterns provides a revised classification of these tumors and has the potential to supply significant biological and clinical insights.