Appearance of albino seedlings and ptDNA inheritance in interspecific hybrids of azalea
Appearance of albino seedlings and ptDNA inheritance in interspecific hybrids of azalea
复制标题
杜鹃花种间杂种白化苗的出现及ptDNA遗传
作者：
K. Ureshino;I. Miyajima;Yukio Ozaki;Nobuo Kobayashi;Ayano Michishita;Masaru Akabane
通过PCR-RFLP分析，明确了（Rhododendron kiusianum × R. eriocarpum） × R. japonicum f. flavum种间三元杂交后代叶片颜色与其ptDNA遗传的关系。所有白化后代都含有母体ptDNA，而绿色和淡绿色后代则含有父亲ptDNA。在培养过程中，叶和茎的颜色由绿色变为白化，在绿色片段中含有母本和父本的ptDNA，在白化片段中含有母本的ptDNA。这些结果表明，白化后代可能是由亲本杂交体质体与黄体核基因组不亲和引起的。
PCR-RFLP analysis was conducted to clarify the relationship between the leaf color of progenies and their ptDNA inheritance in interspecific three-way crosses, (Rhododendron kiusianum × R. eriocarpum) × R. japonicum f. flavum. All albino progenies contained maternal ptDNA, whereas green and pale-green progenies contained paternal ptDNA. Sectorial chimeric progenies, of which the leaf and shoot color was turned from green to albino during the culture, contained both maternal and paternal ptDNA in green segments and maternal ptDNA in albino segments. These results suggest that albino progenies are caused by the incompatibility between plastome from F1 hybrids of R. kiusianum × R. eriocarpum and nuclear genome from R. japonicum f. flavum.