A specific programme of gene transcription in male germ cells
A specific programme of gene transcription in male germ cells
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DOI：
10.1016/s1472-6483(10)61094-2
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发表时间：
2004-05-01
影响因子：
4
通讯作者：
Sassone-Corsi, P
中科院分区：
文献类型：
--
作者：
Kimmins, S;Kotaja, N;Sassone-Corsi, P
雄性生殖细胞的分化需要精子发生阶段和细胞特异性基因表达，这是通过独特的染色质重塑、转录调控和睾丸特异性基因或亚型的表达来实现的。在生殖细胞中发现了协调精子发生分化程序的专门转录复合物，其在一般转录机制的组分中显示出特定的差异。例如，TATA结合（TBP）蛋白家族及其相关辅因子在睾丸中表达上调。在这种生理背景下，转录调控介导的激活CREM代表了一个既定的范例。在体细胞中，CREM的激活需要在一个独特的调控位点（Ser117）磷酸化，随后与无处不在的共激活因子CBR相互作用。在睾丸中，CREM的转录活性是通过与组织特异性伴侣ACT相互作用来控制的，ACT赋予了强大的、磷酸化独立的激活能力。ACT的功能由睾丸特异性驱动蛋白KIF17 b调节。本研究讨论了睾丸特异性转录机制的一些方面，其功能是精子发生过程中必不可少的。
The differentiation of male germ cell requires spermatogenic stage and cell-specific gene expression that is achieved by unique chromatin remodelling, transcriptional control, and the expression of testis-specific genes or isoforms. Specialized transcription complexes that coordinate the differentiation programme of spermatogenesis have been found in germ cells, which display specific differences in the components of the general transcription machinery. The TATA-binding (TBP) protein family and its associated co-factors, for example, show upregulated expression in testis. In this physiological context, transcriptional control mediated by the activator CREM represents an established paradigm. In somatic cells, activation by CREM requires its phosphorylation at a unique regulatory site (Ser117) and subsequent interaction with the ubiquitous coactivator CBR In testis, CREM transcriptional activity is controlled through interaction with a tissue-specific partner, ACT, which confers a powerful, phosphorylation-independent activation capacity. The function of ACT is regulated by a testis-specific kinesin, KIF17b. This study discusses some aspects of the testis-specific transcription machinery, the function of which is essential for the process of spermatogenesis.