Dengue Virus NS1 Protein as a Diagnostic Marker: Commercially Available ELISA and Comparison to qRT-PCR and Serological Diagnostic Assays Currently Used by the State of Florida
Dengue Virus NS1 Protein as a Diagnostic Marker: Commercially Available ELISA and Comparison to qRT-PCR and Serological Diagnostic Assays Currently Used by the State of Florida
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DOI：
10.1155/2017/8072491
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发表时间：
2017-01-01
影响因子：
2.2
通讯作者：
Azizan, Azliyati
中科院分区：
文献类型：
--
作者：
Ambrose, Jason H.;Sekaran, Shamala Devi;Azizan, Azliyati
对感染登革热病毒的患者进行适当管理需要早期发现。在这里，实时分子测定已被证明是有用的，但有局限性，而检测抗体的ELISA仍然是有利的，但结果获得得太晚，没有临床价值。DENV NS 1的产生在感染期间早期达到峰值，并且其检测可以联合收割机结合两种诊断方法的优点。方法.本研究比较了佛罗里达卫生部目前用于检测DENV感染的测定法，包括抗DENV IgM和IgG ELISA以及qRT-PCR，以及市售的DENV NS 1 ELISA。这些比较是在一组21份人血清中进行的。结果14份DENV qRT-PCR+样品中有9份（64.3%）也是DENV NS 1+。有趣的是，qRT-PCR+的5份NS 1-样品另外为IgM-和IgG+，表明非原发性感染。与qRT-PCR相比，NS 1检测的灵敏度为64.3%，特异性为100%，PPV为100%，NPV为58.3%。结论. NS 1 ELISA在已知的DENV qRT-PCR+样品中如预期的那样进行;然而，qRT-PCR+和IgG+血清的阴性NS 1结果似乎降低了NS 1 ELISA对非原发病例的有用性。因此，我们得出结论，通过DENV NS 1 ELISA获得的诊断值得进一步研究。
The proper management of patients infected with dengue virus requires early detection. Here, real-time molecular assays have proven useful but have limitations, whereas ELISAs that detect antibodies are still favored but results are obtained too late to be of clinical value. The production of DENV NS1 peaks early during infection and its detection can combine the advantages of both diagnostic approaches. Methods. This study compared assays currently used for detecting DENV infection at the Florida Department of Health including anti-DENV IgM and IgG ELISAs as well as qRT-PCR, against a commercially available DENV NS1 ELISA. These comparisons weremade among a group of 21 human sera. Results. Nine of 14 (64.3%) DENV qRT-PCR+ samples were also DENV NS1+. Interestingly, the 5 NS1- samples that were qRT-PCR+ were additionally IgM- and IgG+ suggesting a nonprimary infection. Compared to qRT-PCR, the NS1 assay had a sensitivity of 64.3%, specificity 100%, PPV of 100%, and NPV of 58.3%. Conclusions. The NS1 ELISA performed as expected in known DENV qRT- PCR+ samples; however negative NS1 results for qRT-PCR+ and IgG+ sera seemingly reduced the usefulness of the NS1 ELISA for nonprimary cases. We therefore conclude that diagnosis obtained via DENV NS1 ELISA deserves further investigation.