Xenopus Zic4:: Conservation and diversification of expression profiles and protein function among the Xenopus Zic family
Xenopus Zic4:: Conservation and diversification of expression profiles and protein function among the Xenopus Zic family
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DOI：
10.1002/dvdy.20906
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发表时间：
2006-12-01
影响因子：
2.5
通讯作者：
Aruga, Jun
中科院分区：
文献类型：
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作者：
Fujimi, Takahiko J.;Mikoshiba, Katsuhiko;Aruga, Jun
我们比较了非洲爪蟾Zic4与非洲爪蟾Zic家族成员Zic1、Zic2、Zic3和Zic5的表达和功能。Zic4主要在神经板边缘、背神经管和体质体中表达，与Zic1相似，在同一染色体上与Zic4相邻。注射野生型或突变型Zic4 RNA诱导神经嵴标记基因表达，神经组织增生，异位色素细胞形成，表明Zic4与其他爪蟾Zic基因一样，可以诱导神经和神经嵴组织。缺失分析表明，锌指结构域对Zic4的许多功能至关重要，但c端区域对Slug和Sox10两种神经嵴标记的诱导作用不同。动物帽外植体测定的蛋白功能与Zic5相似，但与Zic1、Zic2和Zic3不同，说明非洲爪蟾Zic基因根据功能可分为两类。这些结果表明，尽管非洲爪蟾Zic基因的表达谱和功能存在显著差异，但它们共同调控神经和神经嵴的发育。
We compared the expression and function of Xenopus Zic4 with those of the other four Xenopus laevis Zic family members (Zic1, Zic2, Zic3, and Zic5). Zic4 expression was detected mainly in the neural plate border, dorsal neural tube, and somites, and was similar to that of Zic1, which is adjacent to Zic4 on the same chromosome. Injection of wild-type or mutant Zic4 RNA caused the induction of neural crest marker gene expression, hyperplastic neural tissue, and ectopic pigment cell formation, indicating that Zic4 can induce neural and neural crest tissue, as can other Xenopus Zic genes. Deletion analysis showed that the zinc-finger domain is critical for many Zic4 functions, but the C-terminal region is differently involved in induction of two neural crest markers, Slug and Sox10. The protein function as determined by the animal cap explant assay was similar to that of Zic5, but different from those of Zic1, Zic2, and Zic3, suggesting that Xenopus Zic genes can be divided into two groups based on function. These results indicate that the five Xenopus Zic genes cooperatively regulate both neural and neural crest development, despite significantly diverged expression profiles and functions.