Hepatoblasts comprise a niche for fetal liver erythropoiesis through cytokine production
Hepatoblasts comprise a niche for fetal liver erythropoiesis through cytokine production
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DOI：
10.1016/j.bbrc.2011.05.137
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发表时间：
2011-07-01
影响因子：
3.1
通讯作者：
Okayama, Satoko
中科院分区：
文献类型：
--
作者：
Sugiyama, Daisuke;Kulkeaw, Kasem;Okayama, Satoko
在哺乳动物中，确定性红细胞生成首先发生在胎肝（FL），尽管对该过程如何调节知之甚少。FL由成肝细胞、血窦内皮细胞和造血细胞组成。为了确定胎肝红细胞生成的小生境细胞，我们通过流式细胞术分离每个FL组分。mRNA分析表明，表达Dlk-1的肝母细胞主要表达编码红细胞生成细胞因子的基因EPO和SCF。通过ELISA和免疫组化评估，EPO蛋白主要在肝母细胞中检测到，而在血窦内皮细胞和造血细胞中未检测到。为了表征FL中肝母细胞的功能，我们分析了缺乏肝母细胞的Map 2k 4（-/-）小鼠胚胎，并观察到相对于野生型小鼠，FL中EPO和SCF表达下调。我们的观察表明，肝母细胞包括一个小生境红细胞生成通过细胞因子分泌。(C)2011 Elsevier Inc. All rights reserved.
In mammals, definitive erythropoiesis first occurs in fetal liver (FL), although little is known about how the process is regulated. FL consists of hepatoblasts, sinusoid endothelial cells and hematopoietic cells. To determine niche cells for fetal liver erythropoiesis, we isolated each FL component by flow cytometry. mRNA analysis suggested that Dlk-1-expressing hepatoblasts primarily expressed EPO and SCF, genes encoding erythropoietic cytokines. EPO protein was detected predominantly in hepatoblasts, as assessed by ELISA and immunohistochemistry, and was not detected in sinusoid endothelial cells and hematopoietic cells. To characterize hepatoblast function in FL, we analyzed Map2k4(-/-) mouse embryos, which lack hepatoblasts, and observed down-regulation of EPO and SCF expression in FL relative to wild-type mice. Our observations demonstrate that hepatoblasts comprise a niche for erythropoiesis through cytokine secretion. (C) 2011 Elsevier Inc. All rights reserved.