Adjunctive dental therapies in caries-active children: Shifting the cariogenic salivary microbiome from dysbiosis towards non-cariogenic health.
Adjunctive dental therapies in caries-active children: Shifting the cariogenic salivary microbiome from dysbiosis towards non-cariogenic health.
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DOI：
10.1016/j.humic.2020.100077
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发表时间：
2020-12-01
影响因子：
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通讯作者：
Machida, Curtis A
中科院分区：
文献类型：
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作者：
Lyashenko, Claudia;Herrman, Elisa;Machida, Curtis A
背景技术背景：口腔微生物组是微生物物种的复杂集合，其成分可以使平衡向口腔疾病或持续健康的进展倾斜。最近，我们确定了龋齿活跃和无龋齿儿童唾液微生物组的性别特异性差异。在这项研究中，我们试图确定连续性牙科治疗，包括聚维酮碘和氯己定，是否能有效地将致龋微生物组从生态失调转变为非致龋健康。设计：我们招募年轻的孩子（2-12岁）进入五个招生组，每个组（N = 9-30名参与者/组）接受龋齿修复和/或预防治疗，单独或组合（OHSU IRB #6535）。治疗前后收集唾液标本（4-8周）的龋齿预防措施，并使用下一代测序确定口腔微生物群（HOMINGS，福赛斯研究所，剑桥，MA）。利用多维标度图、支持向量机学习、比值比分析等统计方法，我们已经确定用聚维酮碘治疗可以改变唾液致龋微生物组的组成，使其包括更高比例的需氧微生物，例如嗜麦芽寡养单胞菌，以及非致龋微生物，厌氧微生物包括卟啉单胞菌属和梭杆菌属。我们已经确定了与龋齿活跃儿童相关的微生物，并确定聚维酮碘是一种有效的预防性治疗，有可能将致龋微生物组的组成转变为与非致龋微生物组更接近的组成。生龋健康。
BACKGROUND: The oral microbiome is a complex assembly of microbial species, whose constituents can tilt the balance towards progression of oral disease or sustained health. Recently we identified sex-specific differences in the salivary microbiome contained within caries-active and caries-free children. In this study, we sought to ascertain if adjunctive dental therapies, including povidone iodine and chlorhexidine, were effective in shifting the cariogenic microbiome from dysbiosis to non-cariogenic health.DESIGN: We recruited young children (ages 2-12 years) to enter five enrollment groups, with each group (N = 9-30 participants/group) receiving caries restorative and/or adjunctive therapies, either singularly or in combination (OHSU IRB #6535). Saliva specimens were collected pre- and post-treatment (4-8 weeks) of caries preventive measures, and oral microbiota were identified using next generation sequencing (HOMINGS, Forsyth Institute, Cambridge, MA).RESULTS: With the use of multi-dimensional scaling plots, support vector machine learning, odds ratio analysis, and other statistical methods, we have determined that treatment with povidone iodine can shift the composition of the salivary cariogenic microbiome to include higher proportions of aerobic microorganisms, such as Stentrophomonas maltophila, as well as non-cariogenic, anaerobic microorganisms including Poryphyromonas and Fusobacterium species.CONCLUSION: We have identified microorganisms that are associated with caries-active children and have determined that povidone iodine is an effective adjunctive therapy that has the potential to shift the composition of the cariogenic microbiome to one more closely aligned with non-cariogenic health.