Effect of endophytic fungi on growth of Anoectochilus roxburghii in vitro culture
Effect of endophytic fungi on growth of Anoectochilus roxburghii in vitro culture
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发表时间：
2008
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通讯作者：
Guo Shun-xing
中科院分区：
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作者：
Guo Shun-xing
目的研究真菌AR-15和AR-18对Epuorhiza sp.方法以琼脂为休止剂，研究了真菌AR-15和AR-18对金线莲鲜重和株高的影响；以蛭石为休止剂，研究了真菌和营养物质对金线莲鲜重和株高的影响。结果在琼脂基质中，真菌AR-15和AR-18分别使金线莲幼苗株高增加97%和81.5%(P0.01)；复合处理(真菌AR-15+蒸馏水)极显著地增加了苗木鲜重和株高(P0.01)，分别是未接种的3.26倍和1.51倍。而复合处理(真菌AR-15+营养物质)对苗木鲜重的影响极显著(P0.01)，是对照的1.41倍。复合处理(真菌AR-18+蒸馏水)极显著提高了苗木鲜重和株高(P0.01)，分别是未接种处理的3.86倍和1.45倍。结论真菌AR-15和AR-18对刺梨试管苗的生长有促进作用，而AR-15和AR-18作为生物肥料在刺梨移栽栽培中的应用效果较好。
Objective To study the effect of fungi AR-15 and AR-18(Epulorhiza sp.) on the growth of Anoectochilus roxburghii in vitro culture.Methods Effect of fungi AR-15 and AR-18 on fresh weight and plant height of A.roxburghii were studied in vitro culture,when agar was used as rest,and effect of fungi and nutrients on fresh weight and plant height of A.roxburghii were studied in vitro culture,when vermiculite was used as rest.Results In agar medium,the fungi AR-15 and AR-18 increased the plant height of seedlings by 97% and 81.5%(P0.01).In vermiculite medium,the combination treatment(fungus AR-15+distilled water) extremely significantly increased the fresh weight and plant height of seedlings(P0.01),which was 3.26 and 1.51 times,respectively as compared with the non-inoculated ones.While the effect of combination treatment(fungus AR-15+nutrients) on fresh weight of seedlings was very significant(P0.01),which was 1.41 times as control.And the treatment(fungus AR-15+nutrients) increased significantly the plant height of seedlings by 1.17 times as control(P0.05).In vermiculite medium,the combination treatment(fungus AR-18+distilled water) extremely significantly increased the fresh weight and plant height of seedlings(P0.01),which was 3.86 and 1.45 times,respectively as compared with the non-inoculated ones.While the effect of combination treatment(fungus AR-18+nutrients) on fresh weight of seedlings was very significant(P0.01),which was 1.96 times as control(P0.01).And the treatment(fungus AR-18+nutrients) increased significantly the plant height of seedlings by 1.25 times as control(P0.05).Conclusion The fungi AR-15 and AR-18 could improve the growth of A.roxburghii seedlings in vitro culture.While a good result may be obtained by using the fungi AR-15 and AR-18 as biological manure in transplantation cultivation of A.roxburghii.