Role of neutrophils in hemorrhagic shock-induced gastric mucosal injury in the rat.
Role of neutrophils in hemorrhagic shock-induced gastric mucosal injury in the rat.
复制标题
中性粒细胞在失血性休克引起的大鼠胃粘膜损伤中的作用。
DOI：
10.1016/0016-5085(87)90907-3
复制
发表时间：
1987
期刊：
影响因子：
29.4
通讯作者：
Kvietys,PR
中科院分区：
文献类型：
--
作者：
Smith,SM;Holm-Rutili,L;Perry,MA;Grisham,MB;Arfors,KE;Granger,DN;Kvietys,PR
在30分钟的对照期、30分钟的缺血期（出血至27 mmHg动脉压）和60分钟的再灌注期（回输流出血），测量大鼠胃粘膜对51 Cr标记红细胞（51 Cr-RBC）的清除率。在未处理的大鼠（对照组）中，在再灌注期间观察到51 Cr标记的红细胞渗漏到胃腔中的显著增加。用中性粒细胞抗血清处理可以减弱51 Cr标记的红细胞流入胃腔。使用放射性微球技术，表明嗜中性粒细胞耗尽的动物在缺血期的血流量高于未治疗的大鼠。未处理大鼠放血至平均动脉压为40 mmHg，导致（a）血流量与抗血清处理大鼠流血的至27 mmHg时的血流量无差异，（B）51 Cr标记红细胞渗漏与抗血清处理大鼠相似。本研究结果提示中性粒细胞在失血性休克引起的胃出血中起重要作用。
Gastric mucosal clearance of51Cr-labeled red blood cells (51Cr-RBC) was measured in rats during a 30-min control period, a 30-min ischemic period (hemorrhage to 27 mmHg arterial pressure), and a 60-min reperfusion period (reinfusion of shed blood). In untreated (control) rats, a dramatic rise in the leakage of51Cr-labeled red blood cells into the gastric lumen was observed during the reperfusion period. Treatment with neutrophil antiserum attenuated51Cr-labeled red blood cell flux into the gastric lumen. Using the radioactive microsphere technique, neutrophil-depleted animals were shown to have higher blood flows in the ischemic period than the untreated rats. Bleeding of untreated rats to a mean arterial pressure of 40 mmHg resulted in (a) blood flows that were not different from those in antiserum-treated rats bled to 27 mmHg and (b) leakage of51Cr-labeled red blood cells similar to that measured in antiserum-treated rats. The results of this study indicate that neutrophils play an important role in hemorrhagic shock-induced gastric bleeding.