PROPERTIES OF THE BACTERIOPHAGE-T4 DNA-REPLICATION APPARATUS - THE T4 DDA DNA HELICASE IS REQUIRED TO PASS A BOUND RNA-POLYMERASE MOLECULE
PROPERTIES OF THE BACTERIOPHAGE-T4 DNA-REPLICATION APPARATUS - THE T4 DDA DNA HELICASE IS REQUIRED TO PASS A BOUND RNA-POLYMERASE MOLECULE
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DOI：
10.1016/0092-8674(83)90141-1
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
ALBERTS, BM
中科院分区：
文献类型：
--
作者：
BEDINGER, P;HOCHSTRASSER, M;ALBERTS, BM
DNA复制叉与固定和转录RNA聚合酶分子的相互作用进行了研究，在体外，使用多酶T4噬菌体DNA复制系统和纯化的大肠杆菌RNA聚合酶。当与双链噬菌体fd DNA模板上的启动子结合时，单个固定RNA聚合酶分子可以阻断T4复制叉的移动。当允许转录时（与复制方向相同），复制叉似乎以相对较慢的转录速率跟随移动的RNA聚合酶分子。E.通过加入少量纯化的T4编码的DNA解旋酶（dda基因的产物）消除大肠杆菌RNA聚合酶。显然，含有dda蛋白的复制复合物导致固定的RNA聚合酶分子从DNA中解离。
The interaction of DNA replication forks with both stationary and transcribing RNA polymerase molecules was examined in vitro, using the multienzyme T4 bacteriophage DNA replication system and purified Escherichia coli RNA polymerase. A single stationary RNA polymerase molecule can block the movement of the T4 replication fork when bound to a promoter on a double-stranded phage fd DNA template. When transcription is allowed (in the same direction as replication), the replication fork appears to follow the moving RNA polymerase molecule at the relatively slow rate of transcription. The barriers to fork movement formed by E. coli RNA polymerase are eliminated by the addition of small amounts of a purified T4-encoded DNA helicase, the product of the dda gene. Evidently, replication complexes containing the dda protein cause stationary RNA polymerase molecules to dissociate from the DNA.