Thrombin induces proliferation of osteoblast‐like cells through phosphatidylcholine hydrolysis
Thrombin induces proliferation of osteoblast‐like cells through phosphatidylcholine hydrolysis
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凝血酶通过磷脂酰胆碱水解诱导成骨细胞样细胞增殖
DOI：
10.1002/(sici)1097-4652(199607)168:1
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发表时间：
1996
影响因子：
5.6
通讯作者：
Y. Oiso
中科院分区：
文献类型：
--
作者：
A. Suzuki;O. Kozawa;J. Shinoda;Yasuko Watanabe;H. Saito;Y. Oiso
我们研究了凝血酶对成骨细胞样 MC3T3-E1 细胞中磷脂酰胆碱水解磷脂酶 D 活性的影响。凝血酶在 0.01 至 1 U/ml 范围内剂量依赖性地刺激胆碱的形成，但不刺激磷酸胆碱的形成。二异丙基氟磷酸盐（DFP）灭活的凝血酶对胆碱的形成几乎没有影响。凝血酶和 12-O-十四酰佛波醇-13-乙酸酯（一种蛋白激酶 C 激活佛波醇酯）对胆碱形成的联合作用是相加的。星形孢菌素是一种蛋白激酶抑制剂，对凝血酶诱导的胆碱形成几乎没有影响。联合添加凝血酶和 NaF（异源三聚体 GTP 结合蛋白的激活剂）不会进一步刺激胆碱的形成。百日咳毒素对凝血酶诱导的胆碱形成几乎没有影响。凝血酶刺激Ca2+从细胞外空间流入，时间和剂量依赖性。 EGTA 消耗细胞外 Ca2+ 专门减少了凝血酶诱导的胆碱形成。凝血酶对磷酸肌醇水解磷脂酶 C 活性仅有轻微影响。凝血酶诱导二酰甘油形成和 DNA 合成，并增加 MC3T3-E1 细胞的数量，但 DFP 灭活的凝血酶则不会。凝血酶抑制这些细胞中基础和胎牛血清诱导的碱性磷酸酶活性。普萘洛尔是一种磷脂酸磷酸水解酶抑制剂，可抑制凝血酶诱导的二酰甘油形成和 DNA 合成。这些结果表明，凝血酶由于自身诱导的 Ca2+ 流入而刺激磷脂酰胆碱水解磷脂酶 D，而与成骨细胞样细胞中蛋白激酶 C 的激活无关，并且其增殖作用取决于磷脂酶 D 的激活。 © 1996 Wiley-Liss, Inc.
We examined the effect of thrombin on phosphatidylcholine‐hydrolyzing phospholipase D activity in osteoblast‐like MC3T3‐E1 cells. Thrombin stimulated the formation of choline dose dependently in the range between 0.01 and 1 U/ml, but not the phosphocholine formation. Diisopropylfluorophosphate (DFP)‐inactivated thrombin had little effect on the choline formation. The combined effects of thrombin and 12‐O‐tetradecanoylphorbol‐13‐acetate, a protein kinase C‐activating phorbol ester, on the choline formation were additive. Staurosporine, an inhibitor of protein kinases, had little effect on the thrombin‐induced formation of choline. Combined addition of thrombin and NaF, an activator of heterotrimeric GTP‐binding protein, did not stimulate the formation of choline further. Pertussis toxin had little effect on the thrombin‐induced formation of choline. Thrombin stimulated Ca2+ influx from extracellular space time and dose dependently. The depletion of extracellular Ca2+ by EGTA exclusively reduced the thrombin‐induced choline formation. Thrombin had only a slight effect on phosphoinositide‐hydrolyzing phospholipase C activity. Thrombin induced diacylglycerol formation and DNA synthesis, and increased the number of MC3T3‐E1 cells, but DFP‐inactivated thrombin did not. Thrombin suppressed both basal and fetal calf serum‐induced alkaline phosphatase activity in these cells. Propranolol, an inhibitor of phosphatidic acid phosphohydrolase, inhibited both the thrombin‐induced diacylglycerol formation and DNA synthesis. These results suggest that thrombin stimulates phosphatidylcholine‐hydrolyzing phospholipase D due to self‐induced Ca2+ influx independently of protein kinase C activation in osteoblast‐like cells and that its proliferative effect depends on phospholipase D activation. © 1996 Wiley‐Liss, Inc.
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吲哚美辛抑制凝血酶刺激的胎鼠肢骨吸收，但不抑制甲状腺素刺激的吸收。
DOI：
10.1016/0090-6980(86)90168-1
发表时间：
1986
期刊：
Prostaglandins
影响因子：
--
作者：
Hoffmann,O;Klaushofer,K;Koller,K;Peterlik,M;Mavreas,T;Stern,P
通讯作者：
Stern,P
普萘洛尔是一种磷脂酸磷酸水解酶抑制剂，也能抑制蛋白激酶 C。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sozzani,S;Agwu,DE;McCall,CE;O'Flaherty,JT;Schmitt,JD;Kent,JD;McPhail,LC
通讯作者：
McPhail,LC
DOI：
10.1016/0006-291x(89)91691-4
发表时间：
1989
影响因子：
3.1
作者：
Tatakis,DN;Dolce,C;Dziak,R
通讯作者：
Dziak,R
影响因子：
3.1
作者：
Enright, E;Bland, AP;Kelly, AL
通讯作者：
Kelly, AL