Cellular concentrations of plasmalogen species containing a polyunsaturated fatty acid significantly increase under hypoxia in human colorectal cancer, Caco2 cells
Cellular concentrations of plasmalogen species containing a polyunsaturated fatty acid significantly increase under hypoxia in human colorectal cancer, Caco2 cells
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人类结直肠癌 Caco2 细胞缺氧时，含有多不饱和脂肪酸的缩醛磷脂种类的细胞浓度显着增加
DOI：
10.1016/j.bbrc.2022.04.061
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发表时间：
2022
影响因子：
3.1
通讯作者：
Tokumura Akira
中科院分区：
文献类型：
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作者：
Tamiya-Koizumi Keiko;Otoki Yurika;Nakagawa Kiyotaka;Kannagi Reiji;Mizutani Naoki;Suzuki Motoshi;Kyogashima Mamoru;Iwaki Soichiro;Aoyama Mineyoshi;Murate Takashi;Kitatani Kazuyuki;Kuga Takahisa;Mizutani Yasuyoshi;Tokumura Akira
位于哺乳动物细胞膜筏中的缩醛磷脂在多不饱和脂肪酸（PUFA）的储存中起作用，并且在缺氧条件下存活甚至生长的恶性细胞中以更高的程度存在。据报道哺乳动物细胞中缩醛原的生物合成依赖于有氧条件。使用液相色谱-串联质谱，我们发现，在thesn-2-位置含有PUFA的缩醛磷脂种类的细胞内浓度没有改变，从开始在人结直肠癌衍生的Caco 2细胞缺氧培养两天。缺氧第3天，Caco 2细胞乙醇胺型和胆碱型缩醛磷脂的平均增长率分别为缺氧第2天的2.6倍和2.9倍，这取决于不同的分子种类。在常氧培养，有一个小的数量变化，在任何种类的乙醇胺和胆碱plasmalogens三天。缺氧时非Ca 2+依赖性磷脂酶A2β和胞浆磷脂酶A2γ mRNA的上调以及溶胞浆原酶mRNA的下调可能是缺氧条件下Caco 2细胞缩醛磷脂含量增加的原因。
Plasmalogen localized in the raft of mammalian cell membranes plays a role in the storage of polyunsaturated fatty acid (PUFA), and exists to a higher extent in malignant cells that survive, and even grow in hypoxic conditions. The biosynthesis of plasmalogen in mammalian cells has been reported to depend on aerobic conditions. Using liquid chromatography-tandem mass spectrometry, we found that the intracellular concentration of plasmalogen species containing a PUFA at thesn-2-position did not change for two days from the start of hypoxic culture in human colorectal cancer-derived Caco2 cells. At the third day of hypoxia, Caco2 cells showed the average increase rate of 2.6 times in ethanolamine plasmalogen and 2.9 times in choline plasmalogen depending on the molecular species compared with those in the second day of hypoxia. In normoxic culture, there was little quantitative change in any species of both ethanolamine and choline plasmalogens for three days. The up-regulations of mRNA of Ca2+-independent phospholipase A2β and cytoplasmic phospholipase A2γ as well as the down-regulation of lysoplasmalogenase observed in hypoxia were suggested to be responsible for the increase of plasmalogen in Caco2 cells under hypoxia.