A HOMOLOG OF ESCHERICHIA-COLI RECA PROTEIN IN PLASTIDS OF HIGHER-PLANTS
A HOMOLOG OF ESCHERICHIA-COLI RECA PROTEIN IN PLASTIDS OF HIGHER-PLANTS
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DOI：
10.1073/pnas.89.17.8068
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发表时间：
1992-09-01
影响因子：
11.1
通讯作者：
JAGENDORF, AT
中科院分区：
文献类型：
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作者：
CERUTTI, H;OSMAN, M;JAGENDORF, AT
叶绿体DNA变异的研究和一些直接的实验观察表明，藻类和高等植物质体中存在重组能力。然而，还没有研究所涉及的生化途径。用蓝藻recA基因的一部分作为探针，在Southern印迹中，我们已经发现同源序列的总DNA从豌豆和拟南芥和拟南芥的cDNA文库。克隆并测序了一个cDNA，其预测的氨基酸序列与蓝藻RecA蛋白的氨基酸序列有60.7%的同源性。这一发现是一致的，我们的其他结果表明，DNA链转移活性和存在的蛋白质的预测分子量与抗体大肠杆菌RecA在豌豆叶绿体基质中的交叉反应。
Studies of chloroplast DNA variations, and several direct experimental observations, indicate the existence of recombination ability in algal and higher plant plastids. However, no studies have been done of the biochemical pathways involved. Using a part of a cyanobacterial recA gene as a probe in Southern blots, we have found homologous sequences in total DNA from Pisum sativum and Arabidopsis thaliana and in a cDNA library from Arabidopsis. A cDNA was cloned and sequenced, and its predicted amino acid sequence is 60.7% identical to that of the cyanobacterial RecA protein. This finding is consistent with our other results showing both DNA strand transfer activity and the existence of a protein of the predicted molecular mass crossreactive with antibodies to Escherichia coli RecA in the stroma of pea chloroplasts.