Three-dimensional architecture of rat glomerular basement membrane by ultra-high resolution scanning electron microscopy.
Three-dimensional architecture of rat glomerular basement membrane by ultra-high resolution scanning electron microscopy.
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超高分辨率扫描电子显微镜观察大鼠肾小球基底膜的三维结构。
DOI：
10.18926/amo/30431
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发表时间：
1990
影响因子：
0.5
通讯作者：
Z. Ota
中科院分区：
文献类型：
--
作者：
Y. Yamasaki;H. Makino;K. Hironaka;Y. Hayashi;K. Shikata;Z. Ota
用超高分辨扫描电镜观察了大鼠肾小球基底膜（GBM）的超微结构。通过超声处理方法和导电染色制备的GBM可以在高达400，000倍的放大倍数下观察到没有金属涂层。GBM呈不规则的网状结构，由不同的线和孔组成。链的宽度范围为6至15 nm，孔的直径范围为6至50 nm。本研究证实了我们的分子筛理论的基底膜。
We demonstrated the ultrastructure of rat glomerular basement membrane (GBM) by ultra-high resolution scanning electron microscopy. GBM prepared by sonication methods and conductive-staining could be observed without metal coating at magnifications as high as 400,000 times. The GBM showed an irregular meshwork structure composed of various strands and pores. The width of the strands ranged from 6 to 15 nm, and the diameter of pores ranged from 6 to 50 nm. The present study confirmed our molecular sieve theory of the basement membrane.