Ganoderma lucidum polysaccharides eradicates the blocking effect of fibrinogen on NK cytotoxicity against melanoma cells.
Ganoderma lucidum polysaccharides eradicates the blocking effect of fibrinogen on NK cytotoxicity against melanoma cells.
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DOI：
10.3892/ol.2011.515
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发表时间：
2012-03
期刊：
影响因子：
2.9
通讯作者：
Sheng Zheng;Yanping Jia;Jun Zhao;Qun Wei;Yuehua Liu
中科院分区：
文献类型：
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作者：
Sheng Zheng;Yanping Jia;Jun Zhao;Qun Wei;Yuehua Liu
自然杀伤（NK）细胞的细胞毒性是对血液中转移性肿瘤细胞或病毒的有效防御。然而，NK细胞对肿瘤细胞的细胞毒性可被粘附于肿瘤细胞表面的纤维蛋白原涂层抑制。灵芝（G.灵芝）多糖对I型胶原、透明质酸、纤连蛋白和层粘连蛋白与黑色素瘤细胞上高度表达的整联蛋白的粘附的抑制能力，因此能够增强NK对肿瘤细胞的细胞毒性。本研究探讨了G.灵芝多糖对纤维蛋白原与黑色素瘤细胞粘附及NK细胞对肿瘤细胞杀伤活性的影响。培养黑色素瘤细胞B16和A375并使用流式细胞术分析。分离人NK细胞并使用NK细胞毒性测定法进行分析。结果表明，从灵芝中提取的多糖含量最高。灵芝抑制纤维蛋白原与黑色素瘤细胞的粘附，并逆转纤维蛋白涂层对NK细胞对黑色素瘤细胞的细胞毒作用的阻断作用。
Natural killer (NK) cell cytotoxicity is an effective defense against metastatic tumor cells or viruses in the blood. However, NK cytotoxicity against tumor cells may be inhibited by a fibrinogen coat adhered to the surface of tumor cells. Ganoderma lucidum (G. lucidum) polysaccharides have been reported for their inhibitory ability on the adhesion of type I collagen, hyaluronan, fibronectin and laminin to integrins that were highly expressed on melanoma cells, and were therefore capable of enhancing NK cytotoxicity to tumor cells. In this study, we investigated the effect of G. lucidum polysaccharides on fibrinogen's adhesion to melanoma cells and NK cytotoxicity to tumor cells. Melanoma cells B16 and A375 were cultured and analyzed using flow cytometry. Human NK cells were isolated and analyzed using an NK cytotoxic assay. The results showed that polysaccharides extracted from G. lucidum inhibit the adhesion of fibrinogen to melanoma cells, and reverse the blocking effect of the fibrin coat on NK cytotoxicity against melanoma cells.