Identification and Characterization of Novel Rat Polyomavirus 2 in a Colony of X-SCID Rats by P-PIT assay.
Identification and Characterization of Novel Rat Polyomavirus 2 in a Colony of X-SCID Rats by P-PIT assay.
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DOI：
10.1128/msphere.00334-16
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发表时间：
2016-11
期刊：
影响因子：
4.8
通讯作者：
Chang Y
中科院分区：
文献类型：
--
作者：
Rigatti LH;Toptan T;Newsome JT;Moore PS;Chang Y
虽然P-PIT是为了检测与已知人类多瘤病毒相关的疾病而开发的，但在大鼠中发现一种新的多瘤病毒表明，它可以作为一种广泛筛查新的和已知的多瘤病毒的工具。我们的研究结果表明，RatPyV2可能是实验室大鼠的共生感染，可导致T细胞免疫缺陷大鼠的播散性疾病。X-SCID大鼠感染RatPyV2和卡氏肺囊虫是移植临床前研究中共同感染发病机制和治疗选择的潜在模型。已知多瘤病毒（pyv）可感染多种脊椎动物和无脊椎动物，并与包括癌症在内的多种疾病有关，特别是在免疫抑制宿主中。一种新的多瘤病毒，被命名为大鼠多瘤病毒2 (RatPyV2)，从具有x连锁严重联合免疫缺陷的大鼠繁殖群体中鉴定出来。利用人多瘤病毒免疫组化试验（P-PIT）， RatPyV2最初在一个群体成员的腮腺唾液腺中被检测到。利用来自哈德氏和腮腺的DNA进行Rolling circle扩增，鉴定出一种新的5.1 kb多瘤病毒基因组，与人类华盛顿大学（WU）和卡罗林斯卡研究所（KI）以及田鼠多瘤病毒密切相关，但与褐家鼠PyV1 （RnorPyV1，也称为RatPyV1）明显不同。进一步筛选显示，12/12（100%）的大鼠在肺上皮和其他呼吸、生殖和腺体组织中均有RatPyV2包涵体感染。尽管P-PIT是用于检测与已知人类多瘤病毒相关的疾病，但在大鼠中发现一种新的多瘤病毒表明，它可能作为一种广泛筛查新的和已知多瘤病毒的工具。我们的研究结果表明，RatPyV2可能是实验室大鼠的共生感染，可导致T细胞免疫缺陷大鼠的播散性疾病。X-SCID大鼠感染RatPyV2和卡氏肺囊虫是移植临床前研究中共同感染发病机制和治疗选择的潜在模型。
Although P-PIT was developed to detect diseases associated with known human polyomaviruses, the identification of a new polyomavirus in rats suggests that it may have utility as a broad-based screen for new, as well as known polyomaviruses. Our findings suggest that RatPyV2 may be a commensal infection of laboratory rats that can lead to disseminated disease in T cell immune-deficient rats. Infection of the X-SCID rats with RatPyV2 and Pneumocystis carinii is a potential model for coinfection pathogenesis and treatment options during transplant preclinical studies. Polyomaviruses (PyVs) are known to infect a wide range of vertebrates and invertebrates and are associated with a broad spectrum of diseases, including cancers, particularly in immune-suppressed hosts. A novel polyomavirus, designated rat polyomavirus 2 (RatPyV2), was identified from a breeding colony of rats having X-linked severe combined immunodeficiency. Using a human panpolyomavirus immunohistochemistry test (P-PIT), RatPyV2 was initially detected in the parotid salivary gland of a colony member. Rolling circle amplification using DNA from harderian and parotid glands identified a novel 5.1-kb polyomavirus genome closely related to human Washington University (WU) and Karolinska Institute (KI) and vole polyomaviruses but notably divergent from Rattus norvegicus PyV1 (RnorPyV1; also designated RatPyV1). Further screening showed RatPyV2 inclusion body infection in the lung epithelium and variably in other respiratory, reproductive, and glandular tissues of 12/12 (100%) rats. IMPORTANCE Although P-PIT was developed to detect diseases associated with known human polyomaviruses, the identification of a new polyomavirus in rats suggests that it may have utility as a broad-based screen for new, as well as known polyomaviruses. Our findings suggest that RatPyV2 may be a commensal infection of laboratory rats that can lead to disseminated disease in T cell immune-deficient rats. Infection of the X-SCID rats with RatPyV2 and Pneumocystis carinii is a potential model for coinfection pathogenesis and treatment options during transplant preclinical studies.