Human skin fibroblast collagenase. Assessment of activation energy and deuterium isotope effect with collagenous substrates.
Human skin fibroblast collagenase. Assessment of activation energy and deuterium isotope effect with collagenous substrates.
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DOI：
10.1016/s0021-9258(19)68793-4
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发表时间：
1981-09
期刊：
影响因子：
--
通讯作者：
H. Welgus;J. Jeffrey;A. Eisen
中科院分区：
文献类型：
--
作者：
H. Welgus;J. Jeffrey;A. Eisen
材料与方法试剂：丙烯酰胺和双丙烯酰胺购自伊士曼。从Gallard-Schlesinger中得到十二烷基硫酸钠（纯度为99%）和氧化氘（纯度为99.8%）。Tris碱基、牛胰胰蛋白酶（III型）和大豆胰蛋白酶抑制剂
MATERIALS AND METHODS Reagents-Acrylamide and bis-acrylamide were purchased from Eastman. Sodium dodecyl sulfate (99% pure) and deuterium oxide (99.8%) were obtained from Gallard-Schlesinger. Tris base, bovine pancreatic trypsin (type III), and soybean trypsin inhibitor were