A PROCEDURE FOR RAPID EXTRACTION AND HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC SEPARATION OF THE NUCLEOTIDES AND OTHER SMALL MOLECULES FROM BACTERIAL-CELLS
A PROCEDURE FOR RAPID EXTRACTION AND HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC SEPARATION OF THE NUCLEOTIDES AND OTHER SMALL MOLECULES FROM BACTERIAL-CELLS
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DOI：
10.1016/0003-2697(82)90636-4
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发表时间：
1982-01-01
影响因子：
2.9
通讯作者：
AMES, BN
中科院分区：
文献类型：
--
作者：
PAYNE, SM;AMES, BN
报道了从鼠伤寒沙门氏菌和大肠杆菌中提取和分离核糖核苷和脱氧核糖核苷及核苷酸的方法。通过一种新的快速过滤方法收集细胞，并用甲酸提取小分子。用硼酸亲和层析、反相和离子对高压液相色谱分离酸提取的核苷酸和其他紫外吸收化合物。完整的分析使用了从10毫升对数相细胞中提取的物质，并将小分子池分离成约170种成分。
A method for the extraction and separation of ribo- and deoxyribonucleosides and nucleotides from Salmonella typhimurium and Escherichia coli is described. Cells are harvested by a new rapid filtration method, and the small molecules are extracted with formic acid. The acid-extracted nucleotides and other UV-absorbing compounds are separated by boronate-affinity chromatography and by reverse-phase and ion-pair high-pressure liquid chromatography. The complete analysis uses the extract from 10 ml of log phase cells and separates the small molecule pool into about 170 components.