Optimization of cutaneous electrically mediated plasmid DNA delivery using novel electrode
Optimization of cutaneous electrically mediated plasmid DNA delivery using novel electrode
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DOI：
10.1038/sj.gt.3302867
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发表时间：
2007-02-01
期刊：
影响因子：
5.1
通讯作者：
Heller, R.
中科院分区：
文献类型：
--
作者：
Heller, L. C.;Jaroszeski, M. J.;Heller, R.
皮肤的易接近性使其成为基因转移方案的极好靶点。为了利用皮肤作为基因转移的靶点，重要的是建立一个有效的和可重复的递送系统。电穿孔是用于增强质粒递送至体内许多组织的已建立的技术。该技术的一个关键组成部分是电极配置。电穿孔参数进行了优化的转基因表达与最小的组织损伤与一种新的电极。在100 V/cm的场强下，用8个150 ms脉冲产生最高的转基因表达和单个细胞转化效率，同时损伤最小。这种电极设计提供了比目前市售的简单电极更容易和更可再现的电介导的皮肤质粒递送的潜力。这种电极可以是一个有价值的工具，在确定电介导的皮肤基因转移的适用性。
The easy accessibility of skin makes it an excellent target for gene transfer protocols. To take advantage of skin as a target for gene transfer, it is important to establish an efficient and reproducible delivery system. Electroporation is an established technique for enhancing plasmid delivery to many tissues in vivo. A critical component of this technique is the electrode configuration. Electroporation parameters were optimized for transgene expression with minimal tissue damage with a novel electrode. The highest transgene expression and efficiency of individual cell transformation with minimal damage was produced with eight 150 ms pulses at field strength of 100 V/cm. This electrode design offers the potential for easier and more reproducible electrically mediated cutaneous plasmid delivery than the simple electrodes currently commercially available. This electrode can be a valuable tool in determining the applicability of electrically mediated cutaneous gene transfer.