Effects of a high energy and low protein diet on hepatic and plasma characteristics and Cidea and Cidec mRNA expression in liver and adipose tissue of laying hens with fatty liver hemorrhagic syndrome
Effects of a high energy and low protein diet on hepatic and plasma characteristics and Cidea and Cidec mRNA expression in liver and adipose tissue of laying hens with fatty liver hemorrhagic syndrome
复制标题
高能低蛋白日粮对脂肪肝出血综合征蛋鸡肝脏和血浆特性以及肝脏和脂肪组织中Cidea和Cidec mRNA表达的影响
DOI：
10.1111/asj.13140
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发表时间：
2019-02-01
影响因子：
2
通讯作者：
Huang, Jianzhen
中科院分区：
文献类型：
--
作者：
Peng, Gang;Huang, Enfu;Huang, Jianzhen
Cidea和Cidec是细胞死亡诱导DNA片段因子- α样效应蛋白家族的两个成员，可能参与脂质或脂肪代谢。为进一步了解Cidea和Cidec在脂肪肝出血综合征（FLHS）中的作用，将150只155日龄健康海兰褐蛋鸡随机分为对照组（饲喂基础饲粮）和试验组（饲喂高能量低蛋白[HELP]饲粮）。肝脏组织切片及苏木精和伊红染色分析表明，HELP饲粮可诱导蛋鸡微泡性脂肪变性。随后，根据肝脏颜色评分和组织学检查中观察到的脂质堆积范围，我们将50%的肝脏分类为重度FLHS。结果表明，FLHS患者肝脏和脂肪组织中Cidea和Cidec mRNA表达水平显著升高，且重度FLHS患者肝脏中Cidea和Cidec mRNA表达水平显著高于轻度FLHS患者肝脏。因此，本研究揭示了Cidea和Cidec基因可能参与FLHS形成的途径。
Cidea and Cidec are two members of Cell death-inducing DNA fragmentation factor-alpha-like effector family proteins, which could be involved in lipid or fat metabolism. To better understand the roles of Cidea and Cidec in fatty liver hemorrhagic syndrome (FLHS), 150 healthy 155-day-old Hyline Brown laying hens were randomly divided into control group (fed with basic diet) and experimental group (fed with high-energy low-protein [HELP] diet). Analysis of the liver by tissue sectioning and hematoxylin and eosin staining showed that the HELP diet induced micro-vesicular steatosis in laying hens. Subsequently, based on the liver color scores and the range of lipid accumulation observed in histological examination, we classified livers with 50% as severe FLHS. The results showed that the levels of Cidea and Cidec mRNA expression were markedly elevated in the liver and adipose tissues with FLHS and the levels of Cidea and Cidec mRNA expression in the liver with severe FLHS were significantly higher than that in the liver with mild FLHS. Thus, the present study revealed that the Cidea and Cidec genes may be involved in pathways of FLHS formation.