Overexpression of Nd1-s, a variant form of new kelch family protein, perturbs the cell cycle progression of fibroblasts.
Overexpression of Nd1-s, a variant form of new kelch family protein, perturbs the cell cycle progression of fibroblasts.
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DOI：
10.1089/dna.2005.24.30
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发表时间：
2005-01
影响因子：
3.1
通讯作者：
A. Inoue;Myengmo Kang;L. Fujimura;Yasuyuki Takamori;K. Sasagawa;H. Itoh;T. Tokuhisa;M. Hatano
中科院分区：
文献类型：
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作者：
A. Inoue;Myengmo Kang;L. Fujimura;Yasuyuki Takamori;K. Sasagawa;H. Itoh;T. Tokuhisa;M. Hatano
小鼠 Nd1 基因编码两种形式的蛋白质，Nd1-L 和 Nd1-S，两者共享 BTB/POZ 结构域，但 Nd1-S 缺乏 kelch 重复序列。尽管 Nd1-L 普遍表达、定位于细胞质并作为肌动蛋白丝的稳定剂发挥作用，但 Nd1-S 的表达和功能尚不清楚。在这里，我们表明 Nd1-S 在所有检查的组织中都有表达，并以斑点状模式定位在细胞核中。此外，Nd1-S 的过度表达扰乱了细胞周期 G1/S 期 NIH3T3 细胞的细胞生长。这些结果表明 Nd1-S 可能在细胞核中的细胞周期进程中发挥作用。
The murine Nd1 gene encodes two forms of protein, Nd1-L and Nd1-S, both of which share the BTB/POZ domain, but Nd1-S lacks the kelch repeats. Although Nd1-L ubiquitously expresses, localizes in the cytoplasm and functions as a stabilizer of actin filaments, expression and function of Nd1-S were unknown. Here we show that Nd1-S were expressed in all tissues examined and localized in the nucleus as a speckled-like pattern. Furthermore, overexpression of Nd1-S perturbed cell growth of NIH3T3 cells at the G1/S phase of the cell cycle. These results suggest that Nd1-S may play a role in cell cycle progression in the nucleus.