In vivo and in vitro analysis of Bordetella pertussis catalase and Fe-superoxide dismutase mutants.
In vivo and in vitro analysis of Bordetella pertussis catalase and Fe-superoxide dismutase mutants.
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百日咳博德特氏菌过氧化氢酶和铁超氧化物歧化酶突变体的体内和体外分析。
DOI：
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发表时间：
1996
影响因子：
2.1
通讯作者：
Nicole Guiso
中科院分区：
文献类型：
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作者：
N. Khelef;David DeShazer;Richard L. Friedman;Nicole Guiso
百日咳杆菌产生过氧化氢酶和Fe-超氧化物歧化酶。这些酶的毒力的重要性进行了研究，在体外以及在体内，通过使用突变体在其生产缺陷。过氧化氢酶缺陷型突变体在多形核白细胞内存活，通过细胞凋亡杀死J774A.1巨噬细胞，并在小鼠呼吸道感染模型中表现为亲本菌株。这些结果表明过氧化氢酶在B中没有直接作用。百日咳毒力铁-超氧化物歧化酶表达的缺乏对细菌产生了深远的影响，包括表达腺苷酸环化酶-溶血素和过氧化物酶的能力降低，这两个因素对B很重要。百日咳发病机制铁-超氧化物歧化酶缺陷型突变体在小鼠呼吸道感染模型中的定殖和持续能力也有所下降。
Bordetella pertussis produces a catalase and a Fe-superoxide dismutase. The importance of these enzymes in virulence was investigated, in vitro as well as in vivo, by using mutants deficient in their production. The catalase-deficient mutant survived within polymorphonuclear leukocytes, killed J774A.1 macrophages through apoptosis, and behaved as the parental strain in a murine respiratory infection model. These results suggest no direct role for catalase in B. pertussis virulence. The absence of expression of Fe-superoxide dismutase had profound effects on the bacterium including a reduced ability to express adenylate cyclase-hemolysin and pertactin, two factors important for B. pertussis pathogenesis. The Fe-superoxide dismutase-deficient mutant also had decreased abilities to colonize and persist in the murine respiratory infection model.