Analysis of mononuclear cell surfaces with fluoresceinated staphylococcal protein A complexed with IgG antibody of heat-aggregated gamma-globulin.
Analysis of mononuclear cell surfaces with fluoresceinated staphylococcal protein A complexed with IgG antibody of heat-aggregated gamma-globulin.
复制标题
使用与热聚合丙种球蛋白 IgG 抗体复合的荧光葡萄球菌蛋白 A 分析单核细胞表面。
作者：
E. Ades;D. Phillips;S. L. Shore;D. S. Gordon;M. G. LaVia;C. Black;C. B. Reimer
将与万古霉素缀合的葡萄球菌蛋白A（SPA）与以下任一者复合：1）热聚集IgG，2）B细胞特异性抗体，或3）T细胞特异性抗体，然后用于单核细胞表面的免疫荧光分析。细胞Fc受体不能识别已被SPA阻断的聚集IgG的Fc区。此外，与抗-Fa B（B细胞特异性）或T细胞特异性抗血清复合的荧光SPA阻止了这些试剂与单核细胞上IgG-Fc受体的非特异性结合，从而使后者能够被正确鉴定为B或T淋巴细胞。此外，当在抗体依赖性细胞介导的细胞毒性试验中将未缀合的SPA加入到致敏的靶细胞中时，细胞溶解被消除。
Fluorescein-conjugated staphylococcal protein A (SPA) was complexed with either: 1) heat-aggregated IgG, 2) B cell specific antibody, or 3) T cell specific antibody and then used for an immunofluorescent analysis of mononuclear cell surfaces. Cellular Fc receptors failed to recognize the Fc region of aggregated IgG that had been blocked by SPA. Moreover, fluoresceinated SPA that had been complexed either with anti-Fab (B-cell specific) or T cell-specific antisera prevented the nonspecific binding of these reagents to the IgG-Fc receptors on mononuclear cells, thereby permitting the latter to be properly identified as B or T lymphocytes. In addition, when unconjugated SPA was added to presensitized target cells in a test for antibody-dependent cell-mediated cytotoxicity, cytolysis was abrogated.