Leveraging enzyme structure-function relationships for functional inference and experimental design: The structure-function linkage database
Leveraging enzyme structure-function relationships for functional inference and experimental design: The structure-function linkage database
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DOI：
10.1021/bi052101l
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发表时间：
2006-02-28
期刊：
影响因子：
2.9
通讯作者：
Babbitt, PC
中科院分区：
文献类型：
--
作者：
Pegg, SCH;Brown, SD;Babbitt, PC
对酶进行机械多样化的酶超家族的研究的研究，这些酶进行了不同的总体反应，但共享一个共同的折叠和由关键保守的残基助手执行的独特机械步骤，从而阐明了酶的结构 - 功能关系。我们已经开发了一个资源，即结构功能链接数据库（SFLD），以分析这些结构功能关系。 SFLD独有的是其层次分类方案，基于将特定的部分反应（或其他化学能力）与超家族，亚组和家庭水平保守的特定部分反应（或其他化学能力）与介导它们的保守结构元素的保守。我们介绍了使用SFLD纠正误导，指导蛋白质工程实验的分析结果，并阐明了从结构性基因组学计划中阐明最近解决的酶结构的功能。 SFLD可以在http://sfld.rbvi.ucsf.edu上自由访问。
The study of mechanistically diverse enzyme superfamilies-collections of enzymes that perform different overall reactions but share both a common fold and a distinct mechanistic step performed by key conserved residues-helps elucidate the structure-function relationships of enzymes. We have developed a resource, the structure-function linkage database (SFLD), to analyze these structure-function relationships. Unique to the SFLD is its hierarchical classification scheme based on linking the specific partial reactions (or other chemical capabilities) that are conserved at the superfamily, subgroup, and family levels with the conserved structural elements that mediate them. We present the results of analyses using the SFLD in correcting misannotations, guiding protein engineering experiments, and elucidating the function of recently solved enzyme structures from the structural genomics initiative. The SFLD is freely accessible at http://sfld.rbvi.ucsf.edu.