Glycoprotein isolated from Acanthopanax senticosus protects against hepatotoxicity induced by acute and chronic alcohol treatment
Glycoprotein isolated from Acanthopanax senticosus protects against hepatotoxicity induced by acute and chronic alcohol treatment
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DOI：
10.1248/bpb.29.306
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发表时间：
2006-02-01
影响因子：
2
通讯作者：
Jung, MH
中科院分区：
文献类型：
--
作者：
Choi, JS;Yoon, TJ;Jung, MH
本文研究了刺五加茎皮30 kDa糖蛋白（GF-AS）对急性和慢性酒精性肝损伤的保护作用。GF-AS N端氨基酸序列为NH 2-Val-Ala-Tyr-Pro-Trp-Ala-Gly-Phe-Ala-Leu-Ser-Leu-Glx-Pro-Pro-Ala-Gly-Tyr-。GF-AS显著增加急性酒精处理大鼠的酒精代谢酶，包括酒精脱氢酶、微粒体酒精代谢系统和乙醛脱氢酶的活性，导致血浆酒精水平降低。GF-AS还增加抗氧化酶的活性和谷胱甘肽水平。酒精诱导的肝损伤标志物：急性和慢性治疗大鼠中，GF-AS降低了天冬氨酸转氨酶、丙氨酸转氨酶、甘油三酯和胆固醇的血清水平升高。GF-AS可显著降低慢性酒精处理大鼠的脂肪生成酶，包括苹果酸酶、葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖醛酸脱氢酶的活性。此外，GF-AS还能改善脂肪肝和酒精性肝损害的组织学改变。总的来说，GF-AS可以通过增加乙醇和脂质代谢以及急性和慢性酒精治疗损伤的肝脏中的抗氧化防御系统来减轻酒精诱导的肝毒性。
The protective effect of a 30 kDa glycoprotein (GF-AS) isolated from the stem bark of Acanthopanax senticosus against acute and chronic alcohol-induced hepatotoxicity were studied. N-terminal amino acid sequence of GF-AS showed NH2-Val-Ala-Tyr-Pro-Trp-Ala-Gly-Phe-Ala-Leu-Ser-Leu-Glx-Pro-Pro-Ala-Gly-Tyr-. GF-AS significantly increases the activities of alcohol-metabolizing enzymes, including alcohol dehydrogenase, microsomal ethanol metabolizing system, and acetaldehyde dehydrogenase in rats acutely treated with alcohol, resulting in decreased plasma alcohol levels. GF-AS also increases the activities of antioxidant enzymes and glutathione level. Markers of liver injury induced by alcohol: elevated serum levels of aspartate aminotransferase, alanine aminotransferase, triglyceride and cholesterol, are reduced by GF-AS in both acutely and chronically treated rats. The activities of lipogenic enzymes including malic enzyme, glucose-6-phosphate dehydrogenase, and 6-phosphoglucuronic acid dehydrogenase in chronic alcohol-treated rats are significantly decreased by GF-AS. Furthermore, GF-AS improves histological change in fatty liver and hepatic lesions induced by alcohol. Collectively, GF-AS may alleviate alcohol-induced hepatotoxicity through increasing ethanol and lipid metabolism, as well as antioxidant defence systems in livers injured by acute- and chronic-alcohol treatment.