Evidence for crucial role of hindgut expansion in directing proper migration of primordial germ cells in mouse early embryogenesis
Evidence for crucial role of hindgut expansion in directing proper migration of primordial germ cells in mouse early embryogenesis
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DOI：
10.1016/j.ydbio.2009.04.012
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发表时间：
2009-06-15
影响因子：
2.7
通讯作者：
Kanai, Yoshiakira
中科院分区：
文献类型：
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作者：
Hara, Kenshiro;Kanai-Azuma, Masami;Kanai, Yoshiakira
在小鼠原肠胚形成过程中，原始生殖细胞（PGCs）聚集在尿囊的基部，并在进入生殖嵴之前向尾侧移动进入后肠内胚层。然而，内胚层组织在PGC迁移中的确切作用仍不清楚。通过使用具有特定内胚层缺陷的Sox17突变体，我们为后肠扩张在指导适当的PGC迁移中的关键作用提供了直接证据。在Sox17基因缺失的胚胎中，PGCs通常在尿囊中定殖，然后一小部分前排PGCs将财产转移到最后的肠内胚层。然而，有缺陷的后肠扩张导致PGC进一步侧向运动的失败，导致PGC在后期阶段在后肠入口处的固定。相反，大多数剩余的PGCs移动到内脏内胚层层，但重新定位在胚胎肠道域之外。这导致PGCs分散在胚外卵黄囊内胚层中。这种Sox17无效PGCs的异常迁移可以通过在嵌合胚胎中提供野生型后肠细胞来挽救。因此，这些数据表明，后肠形态发生运动是至关重要的指导PGC运动向胚胎肠道侧，但不是他们的搬迁从中胚层到内胚层。(C)2009 Elsevier Inc. All rights reserved.
During Mouse gastrulation, primordial germ cells (PGCs) become clustered at the base of the allantois and move caudally into the hindgut endoderm before entering the genital ridges. The precise roles of endoderm tissues in PGC migration, however, remain unclear. By using Sox17 mutants with a specific endoderm deficiency, we provide direct evidence for the crucial role of hindgut expansion in directing proper PGC migration. In Sox17-null embryos, PGCs normally colonize in the allantois and then a small front-row population of PGCs moves property into the most posterior gut endoderm. Defective hindgut expansion, however, causes the failure of further lateral PGC movement, resulting in the immobilization of PGCs in the hindgut entrance at the later stages. In contrast, the majority of the remaining PGCs moves into the visceral endoderm layer, but relocate Outside of the embryonic gut domain. This leads to a scattering of PGCs in the extraembryonic yolk sac endoderm. This aberrant migration of Sox17-null PGCs can be rescued by the supply of wildtype hindgut cells in chimeric embryos. Therefore, these data indicate that hindgut morphogenic movement is crucial for directing PGC movement toward the embryonic gut side, but not for their relocation from the mesoderm into the endoderm. (C) 2009 Elsevier Inc. All rights reserved.