Micromachined electrochemical T-switches for cell sorting applications
Micromachined electrochemical T-switches for cell sorting applications
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DOI：
10.1039/b507575k
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发表时间：
2005-01-01
期刊：
影响因子：
6.1
通讯作者：
Liu, CH
中科院分区：
文献类型：
--
作者：
Ho, CT;Lin, RZ;Liu, CH
MEMS微T型开关通过电化学气泡驱动的细胞分选应用在单片芯片级的建议，并成功地证明。研制了一种工作温度低、表面张力大的电解气泡驱动器，用于驱动装置中的微T型开关分选结构。双T结构设计，T形微通道与位于T形微通道的接合处的可移动微T开关结构，以及电解气泡致动器使得有源二元开关功能可用于细胞分选应用。室温操作和电解致动所需的低电压最小化了在常规高电分离仪器（例如流式细胞术）中发生的细胞损伤的可能性。我们的微型T型开关芯片的功能与低所需的驱动电压为3.0类似于3.5 V，本文证明了使用人肝癌细胞。pH值测量表征了致动室和主流微通道中的pH值变化和分布，以追踪电解致动操作期间由于pH值变化而可能导致的肝细胞损伤。通过荧光检测技术，观察到通过我们的微型T开关分选机分选的人肝癌细胞中的细胞存活率为84.1%。此外，70.2%的总注射细胞在分选后在培养物中回收，并在微T开关分选操作后生长成集落。在本文中，我们描述了我们的微T型开关细胞分选芯片的设计，微加工和表征。我们还报告了细胞分选演示和细胞活力的结果，哺乳动物肝细胞通过我们的微T开关细胞分选芯片。
MEMS micro-T-switches actuated via electrochemical bubbles for cell sorting applications in a monolithic chip level are proposed and successfully demonstrated. The electrolysis-bubble actuator, which has the features of low operation temperature and high surface-tension force, is developed to actuate the micro-T-switch sorting structure in our device. The double T-structure design, the T-shape microchannel with the movable micro-T-switch structure located at the junction of the T-shape microchannel, with the electrolysis-bubble actuator makes an active-binary switch function available for cell sorting applications. The room temperature operation and the low voltage required for electrolysis actuation minimize the possibility of cell-damage that happens in the conventional high electric separation instruments, such as flow cytometry. The function of our micro-T-switch chip with a low required actuation voltage of 3.0 similar to 3.5 V is demonstrated by using human hepatoma cells in this paper. The pH-value measurements characterize the pH-value variation and distribution in the actuating chambers and the mainstream microchannels to trace the possible liver-cell injury due to the pH-value variation during electrolysis-actuation operation. The 84.1% cell viability in the sorted human hepatoma cells through our micro-T-switch sorter is observed via the fluorescence assay technique. Furthermore, 70.2% of total injected cells recover in culture after sorting and grow into colonies after micro-T-switch sorting operation. In this paper, we describe the design, microfabrication, and characterization of our micro-T-switch cell-sorting chip. We also report the cell-sorting demonstration and the cell viability results for the mammalian liver cells through our micro-T-switch cell-sorting chip.