Growth Inhibition and Apoptosis of Neuroblastoma Cells Through ROS-Independent MEK/ERK Activation by Sulforaphane
Growth Inhibition and Apoptosis of Neuroblastoma Cells Through ROS-Independent MEK/ERK Activation by Sulforaphane
复制标题
DOI：
10.1007/s12013-013-9522-y
复制
发表时间：
2013-07-01
影响因子：
2.6
通讯作者：
Huang, Tzuu-Yuan
中科院分区：
文献类型：
--
作者：
Hsu, Yi-Chiang;Chang, Sue-Joan;Huang, Tzuu-Yuan
细胞凋亡的失调改变了细胞增殖和细胞死亡的平衡，导致多种疾病，包括癌症。在最近的研究中，萝卜硫素（SFN）已被证明具有有效的抗肿瘤和化学预防活性。SFN诱导人神经母细胞瘤SH-SY 5 Y细胞凋亡的一个可能的信号转导途径也已阐明。本研究通过诱导SH-SY 5 Y细胞凋亡进一步研究SFN的抗增殖活性。我们发现，用SFN处理SH-SY 5 Y细胞导致线粒体膜电位（Δ psi）耗尽，这反过来增加了caspase 9、caspase 3和磷酸化MEK/ERK的上调，而不产生活性氧。结果通过MTT测定证实，其证明SFN对SH-SY 5 Y细胞的细胞毒活性（IC 50值为20 μ M）。
Deregulation of apoptosis alters the balance of cell proliferation and cell death, resulting in a variety of diseases, including cancer. In recent studies, sulforaphane (SFN) has demonstrated potent anti-tumor and chemopreventive activities. A possible signal transduction pathway has also been elucidated for SFN-induced apoptosis in human neuroblastoma SH-SY5Y cells. The present study further investigates the anti-proliferation activities of SFN through induced apoptosis in SH-SY5Y cells. We found that treating SH-SY5Y cells with SFN resulted in the depletion of mitochondrial membrane potential (Delta psi), which in turn increased caspase 9, caspase 3, and the up-regulation of phosphorylated MEK/ERK without generating reactive oxygen species. Results were confirmed by MTT assay, which demonstrated the cytotoxic activity of SFN against SH-SY5Y cells (IC50 values of 20 mu M).