Molecular evolution of poxviruses
Molecular evolution of poxviruses
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DOI：
10.1134/s1022795408080036
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发表时间：
2008-08-01
影响因子：
0.6
通讯作者：
Shchelkunov, S. N.
中科院分区：
文献类型：
--
作者：
Babkin, I. V.;Shchelkunov, S. N.
以前的限制性片段长度多态性分析将天花病毒（VARV）分为两个亚型，其中一个包括西非和南美分离株。这使得一个约会被引入的第一次在估计的VARV演变率。结果被用来分析总科痘病毒的分子进化。比较已知的核苷酸序列进行扩展的保守的中央基因组区域在42个正痘病毒株和多亚基RNA聚合酶的8个基因在65个病毒属于不同属的痘病毒科。使用贝叶斯定年法，痘病毒的突变累积率估计为每年每个位点（1.7-8.8）× 10（-6）个核苷酸替换。计算表明，现代痘病毒属在20多万年前开始从祖先病毒中分离出来，而正痘病毒属的祖先在131 +/- 45千年前出现。具有低GC含量的哺乳动物痘病毒的其他属在大约110-90万年前分化。VARV的独立进化始于3.4 +/- 0.8千年前。以VARV和猴痘病毒（MPXV）为例，这些正痘病毒开始分化进化，随着地理条件的改变，允许西非动物与其他非洲地区隔离，形成了西非VARV和MPXV亚型。
Previous restriction fragment length polymorphism analysis divided variola virus (VARV) strains into two subtypes, one of which included West African and South American isolates. This allowed a dating to be introduced for the first time in estimation of the VARV evolution rate. The results were used to analyze the molecular evolution of the total family Poxviridae. Comparisons of the known nucleotide sequences were performed for the extended conserved central genome region in 42 orthopoxvirus strains and for the eight genes of multisubunit RNA polymerase in 65 viruses belonging to various genera of the family Poxviridae. Using the Bayesian dating method, the mutation accumulation rate of poxviruses was estimated at (1.7-8.8) x 10(-6) nucleotide substitutions per site per year. Computations showed that the modern poxvirus genera started diverging from an ancestral virus more than 200 thousand years ago and that an ancestor of the genus Orthopoxvirus emerged 131 +/- 45 thousand years ago. The other genera of mammalian poxviruses with a low GC content diverged approximately 110-90 thousand years ago. The independent evolution of VARV started 3.4 +/- 0.8 thousand years ago. It was shown with the example of VARV and the monkeypox virus (MPXV) that divergent evolution of these orthopoxviruses started and the West African subtypes of VARV and MPXV were formed as geographical conditions changed to allow isolation of West African animals from other African regions.