Analysis of association between dopamine receptor genes' methylation and their expression profile with the risk of schizophrenia
Analysis of association between dopamine receptor genes' methylation and their expression profile with the risk of schizophrenia
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DOI：
10.1097/ypg.0b013e328363d6e1
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发表时间：
2013-10-01
影响因子：
0.9
通讯作者：
Torkamanzehi, Adam
中科院分区：
文献类型：
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作者：
Kordi-Tamandani, Dor Mohammad;Sahranavard, Roya;Torkamanzehi, Adam
精神分裂症（SCZ）是一种影响大约1%人口的精神障碍。多巴胺是大脑中主要的神经递质之一，其受体与包括SCZ在内的许多精神疾病有关。本研究的目的是分析SCZ患者多巴胺受体DRD1、DRD2、DRD4和DRD5基因的甲基化和表达谱。材料与方法采用甲基化特异性PCR检测80例SCZ患者和71例健康对照者血液样本中DRD1、DRD2、DRD4和DRD5基因启动子甲基化。此外，我们还利用实时反转录PCR技术检测了34份健康对照和病例血液样本中DRD1、DRD2、DRD4和DRD5的mRNA水平。结果与健康对照组相比，病例中DRD4、DRD5和DRD2基因的启动子甲基化具有统计学差异。mRNA表达水平结果也有统计学差异(P
Objective Schizophrenia (SCZ) is a type of psychotic disorder that affects approximate to 1% of the population. Dopamine is one of the major neurotransmitters in the brain and its receptors are associated with a number of psychotic disorders, including SCZ. The aims of the present study were to analyze methylation and the expression profile of dopamine receptor DRD1, DRD2, DRD4, and DRD5 genes in patients with SCZ.Materials and methods Promoter methylation of DRD1, DRD2, DRD4, and DRD5 genes was assayed by a methylation-specific PCR in blood samples obtained from 80 SCZ cases and 71 healthy controls. Also, we investigated DRD1, DRD2, DRD4, and DRD5 mRNA levels using real-time reverse transcription PCR in 34 blood samples of healthy controls and cases.Results Promoter methylation of DRD4, DRD5, and DRD2 genes was statistically different in cases compared with healthy controls. The mRNA expression level results also showed statistically significant differences (P