Presence of corticotropin in human placenta: demonstration of in vitro synthesis.
Presence of corticotropin in human placenta: demonstration of in vitro synthesis.
复制标题
人胎盘中促肾上腺皮质激素的存在：体外合成的演示。
作者：
A. Liotta;R. Osathanondh;K. Ryan;D. Krieger
免疫测定（I）和生物测定（B）ACTH活性存在于广泛洗涤的人胎盘组织和分散的活滋养层（Tr）的提取物中。在选择性剖腹产或阴道分娩的足月胎盘中，以及在妊娠12、15和18周时通过依地兰丁人工流产获得的胎盘中，也存在类似的浓度（I）（1.7-2.7纳克/克）。在分娩前8-48小时内口服地塞米松（8-12 mg）不会显著改变胎盘ACTH含量。B-ACTH浓度是I-ACTH的31-40%。足月胎盘匀浆的Sephadex G-50过滤显示两个I-ACTH峰，一个在空隙体积中洗脱，另一个在合成hACTH 1 -39区域中洗脱。当Tr在组织培养基中孵育时，细胞和培养基的ACTH含量显著高于孵育前的水平。
Immunoassayable (I) and bioassayable (B) ACTH activity is present in extracts of extensively washed human placental tissue and dispersed viable trophoblasts (Tr). Similar concentrations (I) (1.7-2.7 ng/g) are present in term placentas from elective cesarian section or vaginal delivery and from plancentas obtained by prostaglandin-induced abortion at 12, 15 and 18 weeks of gestation. Oral dexamethasone (8-12 mg) administered over an 8-48 h period prior to delivery does not significantly alter placental ACTH content. B-ACTH concentrations are 31-40% of I-ACTH. Sephadex G-50 filtration of term placental homogenates demonstrates two I-ACTH peaks, one eluting in the void volume and the other eluting in the region of synthetic hACTH1-39. When Tr are incubated in tissue culture medium, ACTH content of cells and of medium is significantly greater than pre-incubation levels.