RAPID DIFFERENTIATION OF 4 RELATED SPECIES OF KOJI MOLDS BY AGAROSE-GEL ELECTROPHORESIS OF GENOMIC DNA DIGESTED WITH SMAI RESTRICTION ENZYME
RAPID DIFFERENTIATION OF 4 RELATED SPECIES OF KOJI MOLDS BY AGAROSE-GEL ELECTROPHORESIS OF GENOMIC DNA DIGESTED WITH SMAI RESTRICTION ENZYME
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DOI：
10.2323/jgam.35.225
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发表时间：
1989-06-01
影响因子：
1.2
通讯作者：
TAKAHASHI, K
中科院分区：
文献类型：
--
作者：
GOMI, K;TANAKA, A;TAKAHASHI, K
我们描述了一种简单而快速的方法来区分曲霉、米曲霉和大豆曲霉，以及与之密切相关的黄曲霉和寄生曲霉。曲霉菌株的总DNA用限制性内切酶SmaI酶切，琼脂糖凝胶电泳法分离。用溴化乙锭强烈染色的条带大小在3-5kb之间，种内一致，种间不同。条带大小的差异可能是由于核糖体DNA核苷酸序列的差异所致。在琼脂糖凝胶上可以很容易地区分限制性内切酶的酶切图谱，因此它们将为鉴定曲霉和其他相关物种提供有用的工具。
We describe a simple and rapid procedure for differentiating the koji molds, Aspergillus oryzae and A. sojae, from the closely related species, A. flavus and A. parasiticus. Total DNA from the Aspergillus strains was digested with the restriction enzyme SmaI and was separated by electrophoresis on an agrose gel. The size of the bands, ranging from 3 to 5 kb strongly stained with ethidium bromide, was intraspecifically consistent but was interspecifically different. The difference in the size of the bands seemed to be due to the divergence in the nucleotide sequence of nuclear ribosomal DNA. The restriction enzyme cleavage patterns can be readily distinguished on agarose gel, so they will provide a useful tool for identifying koji molds and other related species.