BIOLOGY OF BLACK LARVAL MUTANT OF TOBACCO HORNWORM, MANDUCA-SEXTA
BIOLOGY OF BLACK LARVAL MUTANT OF TOBACCO HORNWORM, MANDUCA-SEXTA
复制标题
DOI：
10.1016/0022-1910(75)90225-5
复制
发表时间：
1975-01-01
影响因子：
2.2
通讯作者：
RIDDIFORD, LM
中科院分区：
文献类型：
--
作者：
SAFRANEK, L;RIDDIFORD, LM
烟草天蛾Manduca sexta的一个突变体在末龄幼虫中形成黑色黑化角质层。这种黑色表型是由于一个单一的性连锁基因，其表达可以被一个或多个修饰基因改变。突变体表型的表达是防止保幼激素（JH）的应用程序在头帽apolysis在蜕皮周期的最后幼虫龄期。突变体此时对JH的敏感性与颈结扎野生型幼虫相同，排除了激素受体缺失或JH代谢差异的可能性。在这个时候，发现Theblcorpora allata比野生型幼虫活跃，这表明缺陷在于控制的语料库allata。由于选择完全表达的b1表型是容易的，这个突变体提供了一个超灵敏的JH生物测定的基础，将在即将发表的论文中描述。
A mutant of the tobacco hornworm,Manduca sexta, was found to form black melanized cuticle in the last larval instar. Thisblackphenotype is due to a single sex-linked gene whose expression can be changed by one or more modifier genes. The expression of the mutant phenotype is prevented by juvenile hormone (JH) application at the time of head cap apolysis during the moulting cycle to the last larval instar. Theblmutant is equally as sensitive to JH at this time as is a neck-ligated wild type larva, ruling out an absence of hormone receptors or a difference in JH metabolism. Theblcorpora allata were found to be less active at this time than were those of the wild type larva, suggesting that the defect resides in the control of the corpora allata. Since selection for complete expression of theblphenotype is easy, this mutant provides the basis for an ultrasensitive JH bioassay to be described in a forthcoming paper.