Morphofunctional modifications in gill mitochondria-rich cells of Mozambique tilapia transferred from freshwater to 70% seawater, detected by dual observations of whole-mount immunocytochemistry and scanning electron microscopy
Morphofunctional modifications in gill mitochondria-rich cells of Mozambique tilapia transferred from freshwater to 70% seawater, detected by dual observations of whole-mount immunocytochemistry and scanning electron microscopy
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DOI：
10.1016/j.cbpa.2010.09.019
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发表时间：
2011-01-01
影响因子：
2.3
通讯作者：
Kaneko, Toyoji
中科院分区：
文献类型：
--
作者：
Choi, Jeong Hyun;Lee, Kyung Mi;Kaneko, Toyoji
在广盐硬骨鱼莫桑比克罗非鱼 (Oreochromis mossambicus) 中检查了富含线粒体 (MR) 的鳃细胞对从淡水直接转移到 70% 海水的急性反应。扫描电子显微镜（SEM）观察表明，MR细胞的顶端开口在形态上分为顶凹、凸顶面、凹顶面和暂时顶面。同时，在抗Na+/K+-ATP酶（NKA）、T4抗体（检测顶端Na+/Cl-协同转运蛋白（NCC）和基底外侧Na+/K+/2Cl（-）协同转运蛋白（NKCC））和抗Na+/H+交换器-3（NHE3）的整体免疫细胞化学中，NKA免疫反应性MR细胞在功能上被分类为不具有NKCC/NCC的未成熟细胞和 NHE3（I 型）、具有顶端 NCC 的离子吸收细胞（II 型）、具有顶端 NHE3 的细胞（III 型）和具有基底外侧 NKCC 的离子分泌细胞（IV 型）。整体免疫细胞化学和 SEM 的双重观察清楚地显示了 MR 细胞的形态功能改变。转移到70%海水后，具有凸面或凹坑的II型MR细胞关闭其顶端开口以暂停离子吸收。具有凹面或凹坑的 III 型 MR 细胞通过过渡表面转化为具有扩大凹坑的 IV 型 MR 细胞。我们的研究结果表明，III/IV 型 MR 细胞具有切换离子传输功能的功能可塑性，而 II 型 MR 细胞被认为对淡水适应具有特异性。 (C) 2010 Elsevier Inc. 保留所有权利。
Acute responses of gill mitochondria-rich (MR) cells to direct transfer from freshwater to 70% seawater were examined in a euryhaline teleost Mozambique tilapia (Oreochromis mossambicus). Scanning electron microscopic (SEM) observations revealed that apical openings of MR cells were morphologically classified into an apical pit, a convex apical surface, a concave apical surface, and a transitory apical surface. Meanwhile, in whole-mount immunocytochemistry with anti-Na+/K+-ATPase (NKA), T4 antibody (detecting apical Na+/Cl- cotransporter (NCC) and basolateral Na+/K+/2Cl(-) cotransporter (NKCC)), and anti-Na+/H+ exchanger-3 (NHE3), NKA-immunoreactive MR cells were functionally classified into immature cells without both NKCC/NCC and NHE3 (type I), ion-absorptive cells with apical NCC (type II), those with apical NHE3 (type III), and ion-secretory cells with basolateral NKCC (type IV). Dual observations of whole-mount immunocytochemistry and SEM clearly showed morphofunctional alterations in MR cells. After transfer to 70% seawater, type-II MR cells with a convex surface or pit closed their apical openings to suspend ion absorption. Type-III MR cells with a concave surface or pit were transformed into type-IV MR cells with an enlarged pit, via a transitory surface. Our findings indicate functional plasticity of type-III/IV MR cells to switch ion-transport functions, whereas type-II MR cells are considered to be specific for freshwater adaptation. (C) 2010 Elsevier Inc. All rights reserved.