Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
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DOI：
10.1038/nmeth929
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发表时间：
2006-10-01
期刊：
影响因子：
48
通讯作者：
Zhuang, Xiaowei
中科院分区：
文献类型：
--
作者：
Rust, Michael J.;Bates, Mark;Zhuang, Xiaowei
我们开发了一种基于可光开关荧光团的高精度定位的高分辨率荧光显微镜方法。在每个成像周期中，只有一小部分荧光团被打开，从而能够以纳米级的精度确定它们的位置。从一系列成像周期获得的荧光团位置被用来重建整体图像。我们展示了20纳米的成像分辨率。这项技术原则上可以达到分子级的分辨率。
We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.