The developing organ of Corti contains retinoic acid and forms supernumerary hair cells in response to exogenous retinoic acid in culture.
The developing organ of Corti contains retinoic acid and forms supernumerary hair cells in response to exogenous retinoic acid in culture.
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DOI：
10.1242/dev.119.4.1041
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发表时间：
1993-12
期刊：
影响因子：
4.6
通讯作者：
M. Kelley;Xiao-Mei Xu;M. Wagner;M. Warchol;J. Corwin
中科院分区：
文献类型：
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作者：
M. Kelley;Xiao-Mei Xu;M. Wagner;M. Warchol;J. Corwin
Corti的哺乳动物器官具有任何脊椎动物感觉上皮中最高度有序的细胞模式之一。单排内毛细胞和三或四排外毛细胞沿着它的长度延伸。调控这种严格模式形成的因素尚不清楚。为了确定视黄酸是否在Corti器官发育过程中发挥作用，我们在离体小鼠胚胎耳蜗中添加外源性视黄酸。外源性维甲酸显著增加了发育为毛细胞的细胞数量，并导致大面积的多余毛细胞和支持细胞，包括两排内毛细胞和多达11排外毛细胞。维甲酸的作用取决于其浓度和添加时间。Western blot分析表明，胚胎耳蜗感觉上皮中存在细胞维甲酸结合蛋白（CRABP）。从胚胎第14天到出生后第1天，CRABP的含量明显增加，但在成虫的感觉上皮中未检测到CRABP。用维甲酸报告细胞系证实，在胚胎第14天至出生后第1天之间，维甲酸也存在于Corti的发育器官中，并且至少在耳蜗附近也存在于成年耳蜗中。这些结果表明，维甲酸参与了Corti器官的正常发育，维甲酸的作用可能是诱导前感觉细胞群体向毛细胞和支持细胞分化。
The mammalian organ of Corti has one of the most highly ordered patterns of cells in any vertebrate sensory epithelium. A single row of inner hair cells and three or four rows of outer hair cells extend along its length. The factors that regulate the formation of this strict pattern are unknown. In order to determine whether retinoic acid plays a role during the development of the organ of Corti, exogenous retinoic acid was added to embryonic mouse cochleae in vitro. Exogenous retinoic acid significantly increased the number of cells that developed as hair cells and resulted in large regions of supernumerary hair cells and supporting cells containing two rows of inner hair cells and up to 11 rows of outer hair cells. The effects of retinoic acid were dependent on concentration and on the timing of its addition. Western blot analysis indicated that cellular retinoic acid binding protein (CRABP) was present in the sensory epithelium of the embryonic cochlea. The amount of CRABP apparently increased between embryonic day 14 and postnatal day 1, but CRABP was not detectable in sensory epithelia from adults. A retinoic acid reporter cell line was used to demonstrate that retinoic acid was also present in the developing organ of Corti between embryonic day 14 and postnatal day 1, and was also present in adult cochleae at least in the vicinity of the modiolus. These results suggest that retinoic acid is involved in the normal development of the organ of Corti and that the effect of retinoic acid may be to induce a population of prosensory cells to become competent to differentiate as hair cells and supporting cells.